| Author(s): |
Kaushik Chatterjee; Marian F. Young; Carl G. Simon Jr; |
| Title: |
Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture |
| Published: |
May 02, 2011 |
| Abstract: |
Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We describe a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we focus on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels. |
| Citation: |
Methods In Bioengineering |
| Publisher: |
Artech House, Norwood, MA |
| Keywords: |
combinatorial methods; high-throughput screening; hydrogels; elastic modulus; gradients; osteoblast; tissue engineering |
| Research Areas: |
Polymers, Combinatorial Methods, Life Sciences Research, Biomaterials, Biomaterials |
