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Publication Citation: Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture

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Author(s): Kaushik Chatterjee; Marian F. Young; Carl G. Simon Jr;
Title: Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture
Published: May 02, 2011
Abstract: Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We describe a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we focus on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.
Citation: Methods In Bioengineering
Publisher: Artech House, Norwood, MA
Keywords: combinatorial methods; high-throughput screening; hydrogels; elastic modulus; gradients; osteoblast; tissue engineering
Research Areas: Polymers, Combinatorial Methods, Life Sciences Research, Biomaterials, Biomaterials