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|Author(s):||Jaroslaw Grobelny; Frank W. DelRio; Pradeep N. Namboodiri; Doo-In Kim; Vincent A. Hackley; Robert F. Cook;|
|Title:||Size Measurement of Nanoparticles using Atomic Force Microscopy|
|Published:||January 01, 2011|
|Abstract:||This chapter outlines procedures for sample preparation and the determination of nanoparticle size using atomic force microscopy (AFM). To start, procedures for dispersing gold nanoparticles on various surfaces such that they are suitable for imaging and height measurement via intermittent contact mode, or tapping mode, AFM are described. The methods for AFM calibration and operation to make such measurements are then discussed. Finally, the techniques for data analysis and reporting are provided. The example nanoparticles considered are National Institute of Standards and Technology (NIST) Au nanoparticle Reference Materials RM 8011 (nominally 10 nm particles), RM 8012 (nominally 30 nm), and RM 8013 (nominally 60 nm).|
|Citation:||Methods in Molecular Medicine, Characterization of Nanoparticles Intended for Clinical Applications|
|Publisher:||Humana Press, Totowa, NJ|
|Pages:||pp. 71 - 82|
|Keywords:||Nanoparticles, atomic force microscopy|