NIST logo

Publication Citation: Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques

NIST Authors in Bold

Author(s): Marcus T. Cicerone; Joy P. Dunkers; N Washburn;
Title: Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques
Published: January 12, 2004
Abstract: Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.
Citation: Tissue Engineering
Pages: 8 pp.
Keywords: coherence;florescence;microscopy;morphology;OCT;scaffold;tissue engineering
Research Areas: Biomaterials, Tissue Engineering
PDF version: PDF Document Click here to retrieve PDF version of paper (321KB)