| Author(s): |
Daniel W. Bearden; John E. Oatis; Pam Brunsfeld; James W. Rushing; Peter D. Moeller; Thomas N. Gallien; George W. Cooper; |
| Title: |
Isolation, Purification, and Full NMR Assignments of cyclopamine from Veratrum californicum |
| Published: |
June 24, 2009 |
| Abstract: |
The Hedgehog (Hh) signaling pathway is essential for embryogenesis and tissue homeostasis in the adult. However, it may also induce malignancies in a number of tissues when constitutively activated, and it also may have a role in other forms of normal and maladaptive growth in the adult. Cyclopamine, a naturally occurring steroidal alkaloid, is a potent inhibitor of Smoothened, an important response element in the Hh pathway. To use cyclopamine as a tool to explore and/or inhibit the Hh pathway in vivo, a substantial quantity is required, and as a practical matter this has been effectively unavailable. In this paper, we report a rapid and efficient isolation and purification method for large quantities of cyclopamine from the roots and rhizomes of Veratrum californicum Dur. (the Corn Lily or Western false hellebore). |
| Citation: |
Chemistry Central Journal |
| Volume: |
2 |
| Issue: |
12 |
| Keywords: |
Cyclopamine, NMR, Hedgehog pathway, Veratrum californicum, Smoothened |
| Research Areas: |
Drugs/Pharmaceuticals |
