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Publication Citation: Isolation, Purification, and Full NMR Assignments of cyclopamine from Veratrum californicum

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Author(s): Daniel W. Bearden; John E. Oatis; Pam Brunsfeld; James W. Rushing; Peter D. Moeller; Thomas N. Gallien; George W. Cooper;
Title: Isolation, Purification, and Full NMR Assignments of cyclopamine from Veratrum californicum
Published: June 24, 2009
Abstract: The Hedgehog (Hh) signaling pathway is essential for embryogenesis and tissue homeostasis in the adult. However, it may also induce malignancies in a number of tissues when constitutively activated, and it also may have a role in other forms of normal and maladaptive growth in the adult. Cyclopamine, a naturally occurring steroidal alkaloid, is a potent inhibitor of Smoothened, an important response element in the Hh pathway. To use cyclopamine as a tool to explore and/or inhibit the Hh pathway in vivo, a substantial quantity is required, and as a practical matter this has been effectively unavailable. In this paper, we report a rapid and efficient isolation and purification method for large quantities of cyclopamine from the roots and rhizomes of Veratrum californicum Dur. (the Corn Lily or Western false hellebore).
Citation: Chemistry Central Journal
Volume: 2
Issue: 12
Keywords: Cyclopamine, NMR, Hedgehog pathway, Veratrum californicum, Smoothened
Research Areas: Drugs/Pharmaceuticals