Template Fabrication of Protein-Functionalized Gold-Polypyrrole-Gold Segmented Nanowires

Published

September 1, 2004

Author(s)

R M. Hernandez, Lee J. Richter, Stephen Semancik, Stephan J. Stranick, Thomas E. Mallouk

Abstract

Gold nanowires containing protein-modified poly(pyrrole) (Ppy) junctions were synthesized electrochemically using porous aluminum oxide as a template. The effects of the conditions of electrochemical synthesis on Ppy growth and protein (avidin or streptavidin) incorporation were studied. Solution pH had a little effect on protein incorporation; however, higher pH provided slower but more reproducible growth rate of the of Ppy segments. Steptavidin-modified nanowires grown at constant potential had better electrochemical properties and equilibrated faster with fluorescently labeled biotin than did to nanowires grown by potential cycling. The best conditions for synthesis of streptavidin-modified Ppy nanowires were constant potential deposition at 0.75V vs. SCE in a phosphate buffer saline solution at pH 9. This method provides a straightforward route to nanowires of controlled length that can incorporate proteins for use in nano-wire-based biosensors or in nanoparticle assembly through biomolecular interactions.

Citation

Chemistry of Materials

Volume

16

Issue

No. 18

Pub Type

Journals

Keywords

anodic alumina, cyclic voltammetry, gold, nanowire, pH, polypyrrole, protein-functionalization, scanning electron microscopy, transmission electron microscopy

Citation

Hernandez, R. , Richter, L. , Semancik, S. , Stranick, S. and Mallouk, T. (2004), Template Fabrication of Protein-Functionalized Gold-Polypyrrole-Gold Segmented Nanowires, Chemistry of Materials (Accessed April 19, 2024)