| Author(s): |
Rebecca A. Zangmeister; Michael J. Tarlov; |
| Title: |
Selective DNA Sensing Elements Integrated Into Microfluidic Channels |
| Published: |
December 01, 2003 |
| Abstract: |
This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require pre-labeling of the target oligomer prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence of interest as it is electrophoresed through the hydrogel plug sensing platform. The distinct advantages of this assay are the short sensing times as a result of directed electrophoretic transport of target DNA to the sensing element and the ability to detect non-labeled target DNA. |
| Conference: |
MicroTAS 2003 Conference - Seventh International Conference on Micro Total Analysis Systems |
| Proceedings: |
Proceedings of uTAS 2003 Seventh International Conference on Micro Total Analysis Systems |
| Volume: |
2 |
| Pages: |
pp. 1343 - 1346 |
| Location: |
Squaw Valley, CA |
| Dates: |
October 5-9, 2003 |
| Keywords: |
Displacement assay;DNA sensing;Microfluidics;Polyacrylamide hydrogel |
| Research Areas: |
Microfluidics, DNA, Chemistry |
