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Publication Citation: Selective DNA Sensing Elements Integrated Into Microfluidic Channels

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Author(s): Rebecca A. Zangmeister; Michael J. Tarlov;
Title: Selective DNA Sensing Elements Integrated Into Microfluidic Channels
Published: December 01, 2003
Abstract: This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require pre-labeling of the target oligomer prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence of interest as it is electrophoresed through the hydrogel plug sensing platform. The distinct advantages of this assay are the short sensing times as a result of directed electrophoretic transport of target DNA to the sensing element and the ability to detect non-labeled target DNA.
Conference: MicroTAS 2003 Conference - Seventh International Conference on Micro Total Analysis Systems
Proceedings: Proceedings of uTAS 2003 Seventh International Conference on Micro Total Analysis Systems
Volume: 2
Pages: pp. 1343 - 1346
Location: Squaw Valley, CA
Dates: October 5-9, 2003
Keywords: Displacement assay;DNA sensing;Microfluidics;Polyacrylamide hydrogel
Research Areas: Microfluidics, DNA, Chemistry