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NIST Authors in Bold
|Author(s):||Rebecca A. Zangmeister; Michael J. Tarlov;|
|Title:||Selective DNA Sensing Elements Integrated Into Microfluidic Channels|
|Published:||December 01, 2003|
|Abstract:||This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require pre-labeling of the target oligomer prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence of interest as it is electrophoresed through the hydrogel plug sensing platform. The distinct advantages of this assay are the short sensing times as a result of directed electrophoretic transport of target DNA to the sensing element and the ability to detect non-labeled target DNA.|
|Conference:||MicroTAS 2003 Conference - Seventh International Conference on Micro Total Analysis Systems|
|Proceedings:||Proceedings of uTAS 2003 Seventh International Conference on Micro Total Analysis Systems|
|Pages:||pp. 1343 - 1346|
|Location:||Squaw Valley, CA|
|Dates:||October 5-9, 2003|
|Keywords:||Displacement assay,DNA sensing,Microfluidics,Polyacrylamide hydrogel|
|Research Areas:||Microfluidics, DNA, Chemistry|