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|Author(s):||Peter M. Vallone; John M. Butler;|
|Title:||Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations|
|Published:||April 01, 2004|
|Abstract:||Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele-specific primer extension (ASPE) assays and utilized a new commercial allele-specific hybridization (ASH) multiplex kit to examine 50 Y-SNP markers in 229 males from two U.S. Caucasian and African American populations. The novel ASPE assays covered 18 Y-SNP markers in 3 multiplex reactions while a commercial ASH kit was used to type 42 Y-SNPs plus amelogenin for sex-typing purposes. There were 10 overlapping loci between the ASPE and ASH methods permitting an evaluation of concordance on over 2,000 allele calls. The 50 Y-SNP markers examined in this study define 45 of the 159 possible Y Chromosome Consortium (YCC) haplogroups. Only 18 different haplogroups were observed in our samples.|
|Conference:||20th International Congress of the International-Society-for-Forensic-Genetics|
|Proceedings:||International Congress Series; Progress in Forensic Genetics 10|
|Pages:||pp. 85 - 87|
|Dates:||September 9-13, 2003|
|Keywords:||allele-specific hybridization,forensic science,primer extension,single nucleotide polymorphism,SNP typing,Y chromosome,Y-SNPs|
|Research Areas:||Gene Therapy, Genetic Surveillance, DNA, Bioscience & Health|