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Publication Citation: Results from the 1999 NIST Mixed-Stain Study #2 DNA Quantitation, Differential Extraction and Identification of the Unknown Contributors

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Author(s): Margaret C. Kline; Janette W. Redman; David L. Duewer; D J. Reeder;
Title: Results from the 1999 NIST Mixed-Stain Study #2 DNA Quantitation, Differential Extraction and Identification of the Unknown Contributors
Published: September 01, 1999
Abstract: The National Institute of Standards and Technology (NIST) has provided a number of educational interlaboratory exercises to the forensic community. The recent NIST Mixed Stain Study #2 consisted of three separate sets of materials. Set 1) three stains on washed cotton sheeting, representing a sexual assault by an unknown assailant; a blood stain from the known female victim, a neat semen stain from a known suspect, and a mixed blood/semen stain of the above knowns plus an additional unknown semen specimen. Set 2) three stains on washed cotton sheeting, representing a sexual assault; a bloodstain from the known female victim, a neat semen stain from a known suspect, and a mixed blood/semen stain where the semen was different from the male reference. Set 3) five 20 L DNA TE buffer extracts in screw-capped vials with DNA concentrations stated to range from {approximately equal to} 0.2 to {approximately equal to} 20 ng/ L. Two of these Set 3 samples were, in fact, replicates.
Proceedings: 10th International Symposium on Human Identification - 1999
Pages: 12 pp.
Location: Orlando, FL
Dates: September 1, 1999
Keywords: DNA;DNA analysis;DNA concentrations;forensics
Research Areas: Forensics, DNA, Bioscience & Health