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Publication Citation: Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip

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Author(s): Louis P. Hromada; Brian J. Nablo; Michael Gaitan; John J. Kasianowicz; Don DeVoe;
Title: Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip
Published: February 29, 2008
Abstract: A polymer microfluidic platform for electrophysiological ion channel measurements is presented. The system includes an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed. Using this integrated platform, measurements of interactions between membrane-bound ion channels and single poly(ethylene glycol) molecules are demonstrated.
Citation: Lab on A Chip
Pages: pp. 602 - 608
Keywords: electrophysiology;ion channel;microfluidics;on-chip;poly(ethylene glycol);single molecule
Research Areas: Nanobiotechnology
PDF version: PDF Document Click here to retrieve PDF version of paper (333KB)