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|Author(s):||Tasshi Dennis; Shellee D. Dyer; Andrew Dienstfrey; Gurpreet Singh; Paul Rice;|
|Title:||Analyzing quantitative light scattering spectra of phantoms measured with optical coherence tomography|
|Published:||March 01, 2008|
|Abstract:||We demonstrate the ability of multiple forms of optical coherence tomography (OCT) in the frequency domain to quantitatively size scatterers. Combined with a variety of distinct phantoms, we gain insight into the measurement uncertainties associated with using scattering spectra to size scatterers. We size spherical scatterers on a surface using swept source OCT with an analysis based on a simple slab-mode resonance model. Automating this technique, a two-dimensional (2-D) image is created by raster scanning across a surface phantom designed to have a distinct size transition to demonstrate accuracy and repeatability. We also investigate the potential of a novel sphere-nanotube structure as a quantitative calibration artifact for use in comparing measured intensity and phase scattering spectra directly to Mie theory predictions. In another experiment, we demonstrate tissue-relevant sizing of scatterers as small as 5 υ on a surface by use of a Fourier domain OCT system with 280 nm of bandwidth from a super-continuum source. We perform an uncertainty analysis for our high resolution sizing system, estimating a sizing error of 9% for measurements of spheres with a diameter of 15 υ. With appropriate modifications, our uncertainty analysis has general applicability to other sizing techniques utilizing scattering spectra.|
|Citation:||Journal of Biomedical Optics|
|Pages:||pp. 024004-1 - 024004-9|
|Keywords:||dispersion,group delay,Mie,nanotube,optical coherence tomography,phantom,phase,spectral domain|
|PDF version:||Click here to retrieve PDF version of paper (590KB)|