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Publication Citation: Probing Single Nanometer-Scale Pores with Polymeric Molecular Rulers

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Author(s): Sarah E. Henrickson; Edmund A. DiMarzio; Qian Wang; Vincent M. Stanford; John J. Kasianowicz;
Title: Probing Single Nanometer-Scale Pores with Polymeric Molecular Rulers
Published: April 02, 2010
Abstract: It has been shown that individual molecules of single stranded DNA can be driven electrophoretically through a single Staphylococcus aureus ?-hemolysin ion channel. Polynucleotides thread through the channel as extended chains and the polymer-induced ionic current blockades exhibit stable modes during the interactions. Additionally, we show here that polynucleotides can be used to probe structural features of the ?-hemolysin channel itself. Specifically, both pore length and channel aperture profile can be estimated. The results are consistent with the channel crystal structure and suggest that polymer-based ?molecular rulers? may prove useful in deducing the structures of nanometer- scale pores in general.
Citation: Journal of Chemical Physics
Volume: 132
Pages: pp. 135101-1 - 135101-8
Keywords: alpha hemolysin;alpha toxin;DNA;ion channel;nanopore;polymer confinement;polynucleotide;structure-function
Research Areas: Semiconductors
PDF version: PDF Document Click here to retrieve PDF version of paper (948KB)