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|Author(s):||Sarah E. Henrickson; Edmund A. DiMarzio; Qian Wang; Vincent M. Stanford; John J. Kasianowicz;|
|Title:||Probing Single Nanometer-Scale Pores with Polymeric Molecular Rulers|
|Published:||April 02, 2010|
|Abstract:||It has been shown that individual molecules of single stranded DNA can be driven electrophoretically through a single Staphylococcus aureus ?-hemolysin ion channel. Polynucleotides thread through the channel as extended chains and the polymer-induced ionic current blockades exhibit stable modes during the interactions. Additionally, we show here that polynucleotides can be used to probe structural features of the ?-hemolysin channel itself. Specifically, both pore length and channel aperture profile can be estimated. The results are consistent with the channel crystal structure and suggest that polymer-based ?molecular rulers? may prove useful in deducing the structures of nanometer- scale pores in general.|
|Citation:||Journal of Chemical Physics|
|Pages:||pp. 135101-1 - 135101-8|
|Keywords:||alpha hemolysin,alpha toxin,DNA,ion channel,nanopore,polymer confinement,polynucleotide,structure-function|
|PDF version:||Click here to retrieve PDF version of paper (948KB)|