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Topic Area: Life Sciences Research
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Displaying records 11 to 20 of 251 records.
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11. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Topic: Life Sciences Research
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...

12. A vacuum manifold for rapid world-to-chip connectivity of complex PDMS microdevices
Topic: Life Sciences Research
Published: 1/1/2009
Authors: Gregory A Cooksey, Anne L Plant, Francisco Javier Atencia
Abstract: The lack of simple interfaces for microfluidic devices with a large number of inlets significantly limits production and utilization of these devices. In this paper, we describe the fabrication of a reusable manifold that provides rapid world-to-chi ...

Topic: Life Sciences Research
Published: 7/25/1995
Authors: Edward Eisenstein, H, D. Yu, K Fisher, D A Iacuzio, K R Ducote, Frederick P. Schwarz

Topic: Life Sciences Research
Published: 10/1/1995
Authors: Adolfas Kastytis Gaigalas, Joseph Bunton Hubbard, Anne L Plant, Vytautas Reipa

15. Ab Initio Structure of the Active Site of Phosphotriesterase
Topic: Life Sciences Research
Published: 2/1/2001
Author: Morris Krauss
Abstract: This research exploits two recent developments to obtain a fundamental understanding of the metalloenzyme active site using the bi-metallic enzyme phosphotriesterase as an example of this class. First, is the theoretical prediction that the structure ...

16. Accumulation of (5'S)-8,5'-cyclo-2'-deoxyadenosine in organs of Cockayne syndrome complementation group B gene knockout mice
Topic: Life Sciences Research
Published: 2/1/2009
Authors: Guldal Kirkali, N de Souza-Pinto, Pawel Jaruga, V. Bohr, M Miral Dizdar
Abstract: Cockayne syndrome (CS) is a human genetic disorder characterized by sensitivity to UV radiation, neurodegeneration, premature aging among other phenotypes. CS complementary group B (CS-B) gene (csb) encodes the CSB protein (CSB) that is involved in b ...

17. Active Site Structure and Mechanism of Human Glyoxalase I - An Ab Initio Theoretical Study
Topic: Life Sciences Research
Published: 7/25/2001
Authors: U Richter, Morris Krauss
Abstract: The structure of the active site of human glyoxalase I and the reaction mechanism of the enzyme-catalyzed conversion of the thiohemiacetal, formed from methylglyoxal and glutathione, to S-D-lactoylglutathione has been investigated by ab initio quantu ...

18. Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1
Topic: Life Sciences Research
Published: 4/10/2013
Authors: Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant C Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Abstract: Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we p ...

19. Acylation of beta-lactams by class A beta-lactamases: an ab initio theoretical study on the effects of the oxy-anion hole
Topic: Life Sciences Research
Published: 7/9/1997
Authors: B D Wladkowski, S A Chenoweth, J N Sanders, Morris Krauss, W J. Stevens

20. Alpha-Subunit Oxidation in T-State Crystals of a Sebacyl Cross-linked Human Hemoglobin with Unusual Autoxidation Properties
Topic: Life Sciences Research
Published: 1/1/1998
Authors: X Ji, M Karavitis, A. Razynska, H. Kwansa, G B. Vasquez, C Fronticelli, E Bucci, G L. Gilliland

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