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Displaying records 11 to 20 of 151 records.
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11. Best Practices for Evaluating Single Nucleotide Variant Calling Methods for Microbial Genomics
Topic: Life Sciences Research
Published: 7/7/2015
Authors: Nathanael D Olson, Steven P Lund, Rebecca Colman, Jeffery T. Foster, Jason W. Sahl, James M. Schupp, Paul Keim, Jayne B Morrow, Marc L Salit, Justin M Zook
Abstract: Innovations in sequencing technologies have allowed biologists to make incredible advances in understanding biological systems. As experience grows, researchers increasingly recognize that analyzing the wealth of data provided by these new sequen ...

12. CASSCF Investigation of Electronic Excited States of 2-Aminopurine
Topic: Life Sciences Research
Published: 1/11/2001
Authors: E L Rachofsky, J B Ross, Morris Krauss, R Osman
Abstract: 2-aminopurine is a highly fluorescent analog of adenine that can be incorporated synthetically into DNA with little perturbation of the native double-helical structure. The sensitive dependence of the quantum yield of this fluorophore on nucleic aci ...

13. Cell volume distributions reveal cell growth rates and division times
Topic: Life Sciences Research
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...

14. Challenges in Microbial Sampling in the Indoor Environment
Series: Technical Note (NIST TN)
Report Number: 1737
Topic: Life Sciences Research
Published: 3/1/2012
Authors: Jayne B Morrow, Autumn S. Downey, Jordan Peccia
Abstract: This report summarizes the results of the Challenges in Microbial Sampling in the Indoor Environment Workshop held on February 14-15, 2011 at the National Institute of Standards and Technology in Gaithersburg, Maryland.

15. Characterization and Mechanism of Action of Drosophila Ribosomal Protein S3 DNA Glycosylase Activity for the Removal of Oxidatively Damaged DNA Bases
Topic: Life Sciences Research
Published: 12/26/1997
Authors: W. A. Deutsch, A. Yacoub, Pawel Jaruga, T. H. Zastawny, M Miral Dizdar

16. Characterization of DNA Standards by Capillary Electrophoresis
Topic: Life Sciences Research
Published: 2/3/1998
Author: Donald H Atha

17. Characterization of In Vitro Transcription Amplification Linearity and Variability in the Low Copy Number Regime using ERCC Spike Ins
Topic: Life Sciences Research
Published: 10/20/2012
Authors: Jason G Kralj, Marc L Salit
Abstract: Background Characterization of whole transcriptomes from small RNA samples (less than 1 ng total RNA) has been a major challenge. Typically, samples are extracted and purified, amplified by in vitro transcription (IVT), and then subjected to genome- ...

18. Chlorella Virus Pyrimidine Dimer Glycosylase Excises Ultraviolet Radiation- and Hydroxyl Radical-Induced Products 4,6-Diamino-5-Formamidopyrimidine and 2,6-Diamino-4-Hydroxy-5-Formamidopyrimidine from DNA
Topic: Life Sciences Research
Published: 2/1/2002
Authors: Pawel Jaruga, R Jabil, Amanda K McCullough, H Rodriguez, M Miral Dizdar, R Stephen Lloyd
Abstract: A DNA glycosylase specific for UV radiation-induced pyrimidine dimers has been identified from chlorella virus Paramecium bursaria chlorella virus-1. This enzyme (Chlorella virus Pyrimidine dimer glycosylase [cv-pdgl) exhibits a 41% amino acid identi ...

19. Combinatorial Screening of Hydrogel Properties for 3-D Cell Culture: Effect of Stiffness on Encapsulated Osteoblasts
Topic: Life Sciences Research
Published: 7/15/2009
Authors: Carl George Simon Jr., Kaushik Chatterjee, Sheng Lin-Gibson, William E Wallace III, Marian F Young
Abstract: A combinatorial method to rapidly screen cell-material interactions in three-dimensional (3-D) culture format was developed for accelerating tissue engineering research. Hydrogels of poly(ethylene glycol) dimethacrylate (PEGDM) with gradients in comp ...

20. Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture
Topic: Life Sciences Research
Published: 5/2/2011
Authors: Kaushik Chatterjee, Marian F Young, Carl George Simon Jr.
Abstract: Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties ...

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