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Topic Area: Life Sciences Research

Displaying records 61 to 70 of 269 records.
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61. Accumulation of (5'S)-8,5'-cyclo-2'-deoxyadenosine in organs of Cockayne syndrome complementation group B gene knockout mice
Topic: Life Sciences Research
Published: 2/1/2009
Authors: Guldal Kirkali, N de Souza-Pinto, Pawel Jaruga, V. Bohr, M Miral Dizdar
Abstract: Cockayne syndrome (CS) is a human genetic disorder characterized by sensitivity to UV radiation, neurodegeneration, premature aging among other phenotypes. CS complementary group B (CS-B) gene (csb) encodes the CSB protein (CSB) that is involved in b ...

62. A vacuum manifold for rapid world-to-chip connectivity of complex PDMS microdevices
Topic: Life Sciences Research
Published: 1/1/2009
Authors: Gregory A Cooksey, Anne L Plant, Francisco Javier Atencia
Abstract: The lack of simple interfaces for microfluidic devices with a large number of inlets significantly limits production and utilization of these devices. In this paper, we describe the fabrication of a reusable manifold that provides rapid world-to-chi ...

63. Determination of Protein Aggregation with Differential Mobility Analysis: Application to IgG Antibody
Topic: Life Sciences Research
Published: 12/15/2008
Authors: Leonard F. Pease III, John T Elliott, De-Hao D. Tsai, Michael Russel Zachariah, Michael J Tarlov
Abstract: Here we describe the use of electrospray differential mobility analysis (ES-DMA), also known as gas-phase electrophoretic mobility molecular analysis (GEMMA), as a method for measuring low-order aggregates of proteins in solution. We demonstrate pro ...

64. Measurement of formamidopyrimidines in DNA
Topic: Life Sciences Research
Published: 12/15/2008
Authors: Pawel Jaruga, Guldal Kirkali, M Miral Dizdar
Abstract: Formamidopyrimidines, 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua), are among major lesions in DNA generated by hydroxyl radical attack, UV radiation or photosensitization in vitro and in vivo. ...

65. A New Protocol for Semi-Automatic Generation of Reference Data for High Resolution Biological Images
Topic: Life Sciences Research
Published: 11/30/2008
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, James J Filliben
Abstract: We compare manually segmented masks of cell images that are independently hand-selected by several different people, with a new semi-automated method for estimating reference data for images of cells with very sharp clear edges. By quantifying the la ...

66. Soluble Amyloid beta-Oligomers Affect Dielectric Membrane Properties by Bilayer Insertion and Domain Formation: Implications for Cell Toxicity
Topic: Life Sciences Research
Published: 11/15/2008
Authors: Gintaras Valincius, Frank Heinrich, R Budvytye, David J Vanderah, Duncan J. McGillivray, Y Sokolov, J E Hall, Mathias Losche

67. Performance of mitochondrial DNA mutations detecting early stage cancer
Topic: Life Sciences Research
Published: 10/3/2008
Authors: J P. Jakupciak, Samantha D Maragh, Maura E Markowitz, Alissa K Greenberg, Mohammad O Hoque, A Maitra, Peter E. Dr. Barker, Paul D Wagner, William N Rom, Sudhir Srivastava, David Sidransky, Catherine D O'Connell

68. Thermodynamics of the hydrolysis reactions of 1,4-beta-D-xylobiose, 1,4-beta-D-xylotriose, D-cellobiose, and D maltose
Topic: Life Sciences Research
Published: 10/1/2008
Authors: Yadu D. Tewari, Brian E Lang, Stephen R. Decker, Robert Nathan Goldberg
Abstract: Microcalorimetry and high-performance liquid chromatography have been used to conduct a thermodynamic investigation of the following reactions: 1,4--D-xylobiose(aq) + H2O(l) = 2 D-xylose(aq), (1) 1,4--D-xylotriose + 2 H2O(l) = 3 ...

69. The Escherichia coli YdcF binds S-adenosyl-L-methionine and adopts an alpha/beta fold characteristic of nucleotide-utilizing enzymes
Topic: Life Sciences Research
Published: 7/1/2008
Authors: Kinlin L. Chao, Kap Lim, C Lehmann, V Doseeva, A J Howard, Frederick P. Schwarz, Osnat Herzberg
Abstract: The crystal structure of YdcF at a resolution of 0.18 nm reveals a fold of the adenine nucleotide α hydrolase-like family. Following the structural clues, a nucleotide binding screen was carried out employing isothermal titration calorimetry. Th ...

70. Effect of the Distal C162S Mutation on the Energetics of Drug Binding to p38 alpha MAP Kinase
Topic: Life Sciences Research
Published: 1/15/2008
Authors: Niya Todorova, V Doseeva, Jayanthi Ramprakash, Frederick P. Schwarz
Abstract: The binding constant, Kb, and the binding enthalpy (DbHo) of the inhibitor drugs, SB 203580, SKF 86002, and p38 INH.1 binding to two splice variants of p38a MAP kinase and their C162S mutants in 5mM HEPES buffer at pH =7.4 were determined from isoth ...

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