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You searched on: Topic Area: Life Sciences Research

Displaying records 61 to 70 of 142 records.
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61. Standard Reference Material for Her2 Testing: Report of a National Institute of Standards and Technology-Sponsored Consensus Workshop
Topic: Life Sciences Research
Published: 6/1/2003
Authors: M E Hammond, Peter E. Dr. Barker, S Taube, S Gutman
Abstract: A workshop was sponsored by National Institute of Standards and Technology (NIST) Cancer Diagnosis Program of National Cancer Institute (CDP NCI), Food and Drug Administration (FDA), and College of American Pathologists (CAP) to address the need for ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830374

62. Specific Antibody-DNA Interaction: A Novel Strategy for Tight DNA Recognition
Topic: Life Sciences Research
Published: 5/27/2003
Authors: S M Di Pietro, J M Centeno, M L Cerutti, M F Lodeiro, D U Ferreiro, L G Alonso, Frederick P. Schwarz, F Goldbaum, G de Prat-Gay
Abstract: Taking advantage of the extreme stability of a protein-DNA complex, we obtained highly specific monoclonal antibodies against a predetermined palindromic double stranded DNA sequence, corresponding to the binding site of the E2 transcriptional regula ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830182

63. Crystal Structure of the Escherichia Coli YcdX Protein Reveals a Trinuclear Zinc Active Site
Topic: Life Sciences Research
Published: 5/1/2003
Authors: A Teplyakov, G Obmolova, P P Khil, A J Howard, R D Camerini-Otero, G L. Gilliland
Abstract: The ycdX gene of Escherichia coli encodes the protein of 27 kDa molecular weight that belongs to the PHP superfamily of proteins that have diverse functions. This superfamily includes several types of DNA polymerases (namely the a-subunit of bacteria ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830361

64. Comparison of Reagents for Shape Analysis of Fixed Cells by Automated Fluorescence Microscopy
Topic: Life Sciences Research
Published: 4/1/2003
Authors: John T Elliott, Alessandro Tona, Anne L Plant
Abstract: Background: Cell size and shape have been implicated as potentiators intracellular signaling events and as indicator of abnormal cell behavior. Automated microscopy and image analysis can provide quantitative information about the size and shape of ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830369

65. Primary Fibroblasts of Cockayne Syndrome Patients Are Defective in Cellular Repair of 8-Hydroxyguanine and 8-Hydroxyadenine Resulting From Oxidative Stress
Topic: Life Sciences Research
Published: 4/1/2003
Authors: J Tuo, Pawel Jaruga, H Rodriguez, V. Bohr, M Miral Dizdar
Abstract: Cockayne Syndrome (CS) is a genetic human disease with clinical symptoms that include neurodegeneration and premature aging. We show that primary fibroblasts from CS patients lack the ability to efficiently repair oxidative DNA damage. Primary fibr ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830324

66. Arabidopsis Thaliana Ogg1 Protein Excises 8-Hydroxyguanine and 2,6- Diamino-4-hydroxy-5-formamidopyrimidine from Oxidative Damaged DNA Containing Multiple Lesions
Topic: Life Sciences Research
Published: 3/18/2003
Authors: T Morales-Ruiz, M Birincioglu, Pawel Jaruga, H Rodriguez, T. Roldan-Arjona, M Miral Dizdar
Abstract: A functional homologue of eukaryotic Ogg1 proteins in the model plant Arabidopsis thaliana has recently been cloned, isolated, and characterized [Garcia-Ortiz, M.V., Ariza, R.R., and Roldan-Arjona, T. (2001) Plant Mol. Biol. 47, 795-804]. This enzym ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830365

67. Thin Films of Collagen Affect Smooth Muscle Cell Morphology
Topic: Life Sciences Research
Published: 3/4/2003
Authors: John T Elliott, Alessandro Tona, John Taylor Woodward IV, P L Jones, Anne L Plant
Abstract: The purpose of this study was to provide a reproducible method for applying collagen to surfaces on which cells can be grown, and to characterize the resulting thin films of collagen protein with respect to molecular structure and cellular response.C ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830341

68. Oxidative DNA Damage Biomarkers Used in Tissue Engineered Skin
Topic: Life Sciences Research
Published: 1/1/2003
Authors: H Rodriguez, Pawel Jaruga, M Birincioglu, Peter E. Dr. Barker, C D O'Connell, M. Dizdaroglu
Abstract: To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, our laboratory is responding to the need for test methods used to assess the safety and perf ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830346

69. Design and Use of a Peptide Nucleic Acid for Detection of the Heteroplasmic Low-Frequency Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-Like Episodes (MELAS) Mutation in Human Mitochondrial DNA
Topic: Life Sciences Research
Published: 12/1/2002
Authors: Diane K. Hancock, Frederick P. Schwarz, F H Song, L J Wong, Barbara C. Levin
Abstract: Most pathogenic human mitochondrial DNA (mtDNA) mutations are Heteroplasmic and are difficult to detect when present at very low levels in a population of normal mtDNA molecules. This paper describes a simple methodology to detect low levels of the s ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830271

70. Reaction Mechanism of Chorismate Mutase Studied by the Combined Potentials of Quantum Mechanics and Molecular Mechanics
Topic: Life Sciences Research
Published: 11/21/2002
Authors: Y. S. Lee, S. E. Worthington, Morris Krauss, B R Brooks
Abstract: The reaction path for the rearrangement of chorismate to prephenate has been determined in a QM/MM study including the entire protein environment while treating the reaction with ab initio quantum chemistry. In addition to the reactant, chorismate, t ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830322



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