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Topic Area: Life Sciences Research

Displaying records 61 to 70 of 272 records.
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61. Profound asymmetry in the structure of the cAMP-free cAMP receptor protein (CRP) from Mycobacterium tuberculosis
Topic: Life Sciences Research
Published: 3/27/2009
Authors: David Travis Gallagher, N N Smith, Soo-Kyung Kim, Howard Robinson, Prasad T Reddy
Abstract: he bacterial cyclic AMP receptor protein (CRP) has been studied extensively as a model allosterically controlled transcription factor, despite the lack of a crystal structure of its inactive (unliganded) form. We report the crystal structure at 2.0 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832417

62. Cell volume distributions reveal cell growth rates and division times
Topic: Life Sciences Research
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832284

63. Surface plasmon resonance imaging of cells and surface-associated fibronectin
Topic: Life Sciences Research
Published: 2/26/2009
Authors: Alexander W Peterson, Michael W Halter, Alessandro Tona, Kiran Bhadriraju, Anne L Plant
Abstract: Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=902594

64. Accumulation of (5'S)-8,5'-cyclo-2'-deoxyadenosine in organs of Cockayne syndrome complementation group B gene knockout mice
Topic: Life Sciences Research
Published: 2/1/2009
Authors: Guldal Kirkali, N de Souza-Pinto, Pawel Jaruga, V. Bohr, M Miral Dizdar
Abstract: Cockayne syndrome (CS) is a human genetic disorder characterized by sensitivity to UV radiation, neurodegeneration, premature aging among other phenotypes. CS complementary group B (CS-B) gene (csb) encodes the CSB protein (CSB) that is involved in b ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832387

65. A vacuum manifold for rapid world-to-chip connectivity of complex PDMS microdevices
Topic: Life Sciences Research
Published: 1/1/2009
Authors: Gregory A Cooksey, Anne L Plant, Francisco Javier Atencia
Abstract: The lack of simple interfaces for microfluidic devices with a large number of inlets significantly limits production and utilization of these devices. In this paper, we describe the fabrication of a reusable manifold that provides rapid world-to-chi ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901151

66. Determination of Protein Aggregation with Differential Mobility Analysis: Application to IgG Antibody
Topic: Life Sciences Research
Published: 12/15/2008
Authors: Leonard F. Pease III, John T Elliott, De-Hao D. Tsai, Michael Russel Zachariah, Michael J Tarlov
Abstract: Here we describe the use of electrospray differential mobility analysis (ES-DMA), also known as gas-phase electrophoretic mobility molecular analysis (GEMMA), as a method for measuring low-order aggregates of proteins in solution. We demonstrate pro ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832275

67. Measurement of formamidopyrimidines in DNA
Topic: Life Sciences Research
Published: 12/15/2008
Authors: Pawel Jaruga, Guldal Kirkali, M Miral Dizdar
Abstract: Formamidopyrimidines, 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua), are among major lesions in DNA generated by hydroxyl radical attack, UV radiation or photosensitization in vitro and in vivo. ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832358

68. A New Protocol for Semi-Automatic Generation of Reference Data for High Resolution Biological Images
Topic: Life Sciences Research
Published: 11/30/2008
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, James J Filliben
Abstract: We compare manually segmented masks of cell images that are independently hand-selected by several different people, with a new semi-automated method for estimating reference data for images of cells with very sharp clear edges. By quantifying the la ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904743

69. Soluble Amyloid beta-Oligomers Affect Dielectric Membrane Properties by Bilayer Insertion and Domain Formation: Implications for Cell Toxicity
Topic: Life Sciences Research
Published: 11/15/2008
Authors: Gintaras Valincius, Frank Heinrich, R Budvytye, David J Vanderah, Duncan J. McGillivray, Y Sokolov, J E Hall, Mathias Losche
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906576

70. Performance of mitochondrial DNA mutations detecting early stage cancer
Topic: Life Sciences Research
Published: 10/3/2008
Authors: J P. Jakupciak, Samantha D Maragh, Maura E Markowitz, Alissa K Greenberg, Mohammad O Hoque, A Maitra, Peter E. Dr. Barker, Paul D Wagner, William N Rom, Sudhir Srivastava, David Sidransky, Catherine D O'Connell
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906440



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