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You searched on: Topic Area: Life Sciences Research

Displaying records 61 to 70 of 153 records.
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61. Importance of Product/ Reactant Equilibrium in the Kinetics of the Phosphoglucose Isomerization Reaction by Differential Stopped Flow Microcalorimetry
Topic: Life Sciences Research
Published: 6/15/2004
Authors: M Stodeman, Frederick P. Schwarz
Abstract: The kinetics for the isomerization of fructose-6-phosphate to glucose-6-phosphate (F6P -> G6P) by baker's yeast phosphoglucose isomerase in terms of k^dcat^ and K^dm^ was determined from analysis of Differential Stopped Flow Microcalorimeter (DSFM) m ...

62. Temperature-induced structural changes in putidaredoxin: a circular dichroism and UV-VIS absorption study
Topic: Life Sciences Research
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker

63. Structure and Reaction in the Active Site of Mammalian Adenylyl Cyclase
Topic: Life Sciences Research
Published: 4/8/2004
Authors: Y. S. Lee, Morris Krauss
Abstract: The reaction path for the catalytic conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP) by the enzyme mammalian adenylyl cyclase has been calculated theoretically using the Hartree-Fock method. The crystal structure of ...

64. Crystal Structure of the Complex Between Thrombin and the Central "E" Region of Fibrin
Topic: Life Sciences Research
Published: 3/2/2004
Authors: I Pechik, J Madrazo, M W Mosesson, I Hernandez, G L. Gilliland, L Medved
Abstract: Non-substrate interactions of thrombin with fibrin play an important role in modulating its procoagulant activity. To establish the structural basis for these interactions, we crystallized PPACK-inhibited thrombin in complex with a fragment, Eht , c ...

65. Overexpression and Rapid Purification of Escherichia Coli Formamidopyrimidine-DNA Glycosylase
Topic: Life Sciences Research
Published: 3/1/2004
Authors: Prasad T Reddy, Pawel Jaruga, T R O'Connor, H Rodriguez, M Miral Dizdar
Abstract: Formamidopyrimidine DNA glycosylase (Fpg) is a DNA glycosylase with an associated AP lyase activity. As a DNA repair enzyme, Fpg excises several modified bases from DNA associated with exposure to oxidizing agents such as free radicals. Experiments ...

66. Membrane Surface Dynamics of DNA-Threaded Nanopores Revealed by Simultaneous Single-Molecule Optical and Ensemble Electrical Recording
Topic: Life Sciences Research
Published: 2/3/2004
Authors: E L Chandler, A L Smith, Lisa K Burden, John J Kasianowicz, D L Burden
Abstract: We demonstrate a method for simultaneous real-time electrical and single-molecule optical recordings of the interactions between single-stranded DNA and nanoscopic pores in planar lipid membranes. Electrophysiological techniques are used to measure ...

67. Substrate specificities and excision kinetics of DNA glycosylases involved in base-excision repair of oxidative DNA damage
Topic: Life Sciences Research
Published: 10/29/2003
Author: M Miral Dizdar

68. Temperature Dependence of the Kinetics of the Acylase Hydrolysis Reaction by Differential Stopped Flow Microcalorimetry
Topic: Life Sciences Research
Published: 10/1/2003
Authors: M Stodeman, Frederick P. Schwarz
Abstract: The rate of the hydrolysis of N-acetyl-L-methionine by porcine acylase I in 0.1 M phosphate buffer, which is reduced by acetate product inhibition, was monitored calorimetrically at 288.4 K, 298.4 K, 308.5 K, and 311.5 K by a differential stopped flo ...

69. DNA Base Damage by the Antitumor Agent 3-Amino-1,2,4-Benzotriazine 1,4-Dioxide (Tirapazamine)
Topic: Life Sciences Research
Published: 9/24/2003
Authors: M Birincioglu, Pawel Jaruga, G Chowdhury, H Rodriguez, M Miral Dizdar, K Gates
Abstract: Tirapazamine is a bioreductively activated DNA-damaging agent that selectively kills the hypoxic cells found in solid tomors. This compound shows great clinical promise and is currently being examined in a variety of clinical trails, including sever ...

70. Crystal Structure of the YchF Protein Reveals Binding Sites for GTP and Nucleic Acid
Topic: Life Sciences Research
Published: 7/1/2003
Authors: A Teplyakov, G Obmolova, S Y Chu, J Toedt, E Eisenstein, A J Howard, G L. Gilliland
Abstract: The bacterial protein encoded by the gene ychF is 1 of 11 universally conserved GTPases, and the only one whose function is unknown. The crystal structure determination of YchF was sought to help with the the functional assignment of the protein. ...

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