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Topic Area: Bioscience
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Displaying records 401 to 410 of 467 records.
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401. Synchrotron White-Beam X-Ray Topography of Ribonuclease S Crystals
Topic: Bioscience & Health
Published: 4/1/2002
Authors: W M Vetter, David Travis Gallagher, M Dudley
Abstract: With careful experimental design indexed synchrotron white-beam X-ray topographs of ribonuclease S crystals at ambient temperature could be recorded with a definition and contrast comparable to that of monochromatic beam topographs of other proteins ...

402. Synthetic spike-in standards for RNA-seq experiments
Topic: Bioscience & Health
Published: 9/1/2011
Authors: Marc L Salit, Lichun Jiang, Felix Schlesinger,, Brian Oliver, Carrie Davis, Yu Zhang, Renhua Li, Thomas R Gingeras
Abstract: High-throughput sequencing of cDNA (RNA-seq) is a widely deployed transcriptome profiling and annotation technique, but questions about the performance of different protocols and platforms remain. We used a newly developed pool of 96 synthetic RNAs w ...

403. Synthetic spike-in standards improve run-specific systematic error analysis for DNA and RNA sequencing
Topic: Bioscience & Health
Published: 7/31/2012
Authors: Justin M Zook, Daniel Victor Samarov, Jennifer H McDaniel, Shurjo Sen, Marc L Salit
Abstract: While the importance of random sequencing errors decreases at higher DNA or RNA sequencing depths, systematic sequencing errors (SSEs) dominate at high sequencing depths and can be difficult to distinguish from biological variants. These SSEs can ...

404. T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements
Topic: Bioscience & Health
Published: 4/7/2009
Authors: Jason G Kralj, Audrey Player, Hope Sedrick, Matthew S Munson, David Petersen, Samuel P Forry, Paul Meltzer, Ernest Kawasaki, Laurie E Locascio
Abstract: We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microa ...

405. TLR stimulation initiates a CD39-based autoregulatory mechanism that limits macrophage inflammatory responses
Topic: Bioscience & Health
Published: 9/12/2013
Authors: John P Marino, Katharine Therese Briggs, Heather Cohen, Katya Ravid, Simon C. Robson, David M. Mosser
Abstract: Sepsis is a highly fatal disease caused by an initial hyperinflammatory response followed by a state of profound immunosuppression. Although it is well appreciated that the initial production of proinflammatory cytokines by macrophages accompanies th ...

406. Tandem Isotachophoresis-Zone Electrophoresis via Base-Mediated Destacking for Increased Detection Sensitivity in Microfluidic Systems
Topic: Bioscience & Health
Published: 5/21/2003
Authors: Wyatt N Vreeland, S J Williams, A E Barron, A P Sassi

407. Tandem Mass Spectral Libraries of Peptides in Digests of Individual Proteins: Human Serum Albumin
Topic: Bioscience & Health
Published: 6/2/2014
Authors: Qian Dong, Xinjian Yan, Lisa Ervin Kilpatrick, Yuxue Liang, Yuri Alexander Mirokhin, Jeri S Roth, Stephen E Stein
Abstract: This work presents a method for creating a mass spectral library containing tandem spectra identifiable peptide ions in the tryptic digestion of a single protein. Human serum albumin (HSA) was selected for this purpose owing to its ubiquity and h ...

408. Technical Assessment of Test and Evaluation Supporting Infrastructure for BioWatch. Summary Report from Peer Review of the Los Alamos National Laboratory.
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7882
Topic: Bioscience & Health
Published: 11/19/2012
Authors: Jayne B Morrow, David A LaVan

409. Technical Assessment of Test and Evaluation Supporting Infrastructure for BioWatch. Summary Report from Peer Review of the Massachusetts Institute of Technology - Lincoln Laboratory
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7881
Topic: Bioscience & Health
Published: 11/19/2012
Authors: Jayne B Morrow, David A LaVan

410. Teeth: Among Nature's Most Durable Biocomposites
Topic: Bioscience & Health
Published: 4/6/2010
Authors: Brian Ronald Lawn, James J. Lee, H Chai
Abstract: This paper addresses the durability of natural teeth from a materials perspective. Teeth are depicted as 'smart' biocomposites, highly resistant to cumulative deformation and fracture. Favorable morphological features of teeth at both mac ...

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