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Author: Eric Kilpatrick
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1. Expression and characterization of 15N-labeled human C-reactive protein in Escherichia coli and Pichia pastoris for use in isotope-dilution mass spectrometry
Eric L Kilpatrick, Wei-Li Liao, Johanna Camara, Illarion V Turko, David M Bunk
Isotope dilution mass spectrometry using stable isotope labeled internal standards is unsurpassed in its ability to quantify analytes with accuracy and low measurement uncertainty. The extension of this technique to proteins relies upon the expressi ...
2. Multiple forms of Selenoprotein P in a Candidate Human Plasma Standard Reference Material
William C Davis, Lisa Ervin Kilpatrick, Eric L Kilpatrick, Guillaume Ballihaut
Selenoprotein P (SePP) is a unique selenium-containing protein responsible for the transport and distribution of the essential trace element selenium (Se) through the human body with the concentration of SePP in human blood representing the most usef ...
3. EVALUATION OF A MICROWAVE-ASSISTED METAL-LABELING STRATEGY FOR QUANTIFICATION OF PEPTIDES VIA RPLC-ICP-MS AND THE METHOD OF STANDARD ADDITIONS
Steven J Christopher, Eric L Kilpatrick, Lee Lijian Yu, William C Davis, blakely adair
NIST has performed preliminary research on applying a calibration methodology based on the method of standard additions to the quantification of lanthanide-labeled peptides via reverse-phase liquid chromatography coupled to inductively coupled plasma ...
4. Detection and Characterization of Selenoproteins by Tandem Mass Spectrometry Assisted by Laser Ablation Inductively Coupled Plasma Mass Spectrometry: Application to Human Plasma Selenoproteins
Guillaume Ballihaut, Lisa Ervin Kilpatrick, Eric L Kilpatrick, William C Davis
There has been an on-going effort and long term goal to accurately assess selenium status and selenium nutritional requirement in humans through the quantification of human plasma selenoprotein P (SelP) and glutathione peroxidase 3 (GPx3). To achiev ...
5. Reference Measurement Procedure Development for C-Reactive Protein in Human Serum
Eric L Kilpatrick, David M Bunk
C-reactive protein (CRP) is a serum analyte of clinical interest in ranges which are difficult to detect using standard mass spectrometry approaches. This paper describes a measurement procedure utilizing affinity purification of intact CRP prior to ...