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Author: laurie locascio
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Displaying records 81 to 90 of 97 records.
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81. Reagentless Cleavage of the Peptide Backbone Using Light-Generated Radicals
Published: Date unknown
Authors: Barbara Jo Jones, Mathew J. Vergne, David M Bunk, Laurie E Locascio, M A Hayes
Abstract: A key step for protein identification and characterization is cleavage into distinct fragments. All current cleavage methods require the addition of reagents, either proteolytic enzymes or chemical agents, and often a second reagent to discontinue c ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832122

82. Room-Temperature Imprinting Method for Plastic Microchannel Fabrication
Published: 4/15/2000
Authors: J. Xu, Laurie E Locascio, Michael Gaitan, C G Lee
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=27951

83. Semiconductor nanocrystal conjugates, FISH and pH
Published: 10/1/2005
Authors: Yan Xiao, W G Telford, J C Ball, Laurie E Locascio, Peter E. Dr. Barker
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903222

84. Simulation and experimental characterization of electroosmotic flow in surface modified channels
Published: 7/1/2006
Authors: S. Krishnamoorthy, J. Feng, A. C. Henry, Laurie E Locascio, J. Hickman, S. Sundaram
Abstract: Covalent surface modification techniques, in particular surface oxidation procedures, have been employed as a mean to modify polymer microfluidic channels for the purpose of modulating microflow. The focus of this work is to experimentally and comput ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908138

85. Simultaneous Concentration and Separation of Coumarins Using a Molecular Micelle in Micellar Affinity Gradient Focusing
Published: 3/1/2007
Authors: Mary W Kamande, David J Ross, Laurie E Locascio, Mark Lowry, Isiah M Warner
Abstract: We report the use of a molecular micelle for the simultaneous separation and concentration of neutral and hydrophobic analytes using micellar affinity gradient focusing (MAGF). The technique, MAGF, combines the favorable features of micellar electrok ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832146

86. Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
Published: 12/1/2011
Authors: Justin M Zook, Robert I. MacCuspie, Laurie E Locascio, Michael W Halter, John T Elliott
Abstract: Methods to disperse nanoparticles reproducibly in aqueous solutions compatible with cell-based assays are critical for studying cytotoxicity. Here, we disperse gold and silver nanoparticles in cell culture media, letting them agglomerate to a variet ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906154

87. Surface Characterization of Laser- Ablated Polymers Used for Microfluidics
Published: 2/15/2002
Authors: D L Pugmire, E A. Waddell, R Haasch, Michael J Tarlov, Laurie E Locascio
Abstract: Fabrication of microfluidic devices by excimer laser ablation under different atmospheres may provide variations in polymer microchannel surface characteristics. The surface chemistry and electroosmotic (EO) mobility of polymer microchannels laser a ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830754

88. Surface Modification of PMMA for Application of Wall Coating Polymers for Microchip Electrophoresis
Published: 9/1/2006
Authors: Jayna J Shah, Jon C Geist, Laurie E Locascio, Michael Gaitan, Rao Mulpuri, Wyatt N Vreeland
Abstract: The development of rapid and simple wall coating strategies for high resolution electrophoretic separation of DNA is of crucial importance for the successful implementation of miniaturized polymeric DNA analysis systems. In this report, we present an ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=32214

89. T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements
Published: 4/7/2009
Authors: Jason G Kralj, Audrey Player, Hope Sedrick, Matthew S Munson, David Petersen, Samuel P Forry, Paul Meltzer, Ernest Kawasaki, Laurie E Locascio
Abstract: We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microa ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832379

90. Temperature Measurement and Control in Microfluidic Systems
Published: 10/1/2001
Authors: David J Ross, Michael Gaitan, Laurie E Locascio
Abstract: We describe a technique for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. The technique is easy to implement with a standard fluorescence microscope and CCD camera. The efficiency of the me ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830750



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