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Author: laurie locascio
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81. Quantum Dot FRET-Based Probes in Thin Films Grown in Microfluidic Channels
Georgeta Crivat, Sandra Maria Da Silva, Darwin R Reyes-Hernandez, Laurie E Locascio, Michael Gaitan, Nitsa Rosenzweig, Zeev Rosenzweig
82. Reagentless Cleavage of the Peptide Backbone Using Light-Generated Radicals
Barbara Jo Jones, Mathew J. Vergne, David M Bunk, Laurie E Locascio, M A Hayes
A key step for protein identification and characterization is cleavage into distinct fragments. All current cleavage methods require the addition of reagents, either proteolytic enzymes or chemical agents, and often a second reagent to discontinue c ...
83. Room-Temperature Imprinting Method for Plastic Microchannel Fabrication
J. Xu, Laurie E Locascio, Michael Gaitan, C G Lee
84. Semiconductor nanocrystal conjugates, FISH and pH
Yan Xiao, W G Telford, J C Ball, Laurie E Locascio, Peter E. Dr. Barker
85. Simulation and experimental characterization of electroosmotic flow in surface modified channels
S. Krishnamoorthy, J. Feng, A. C. Henry, Laurie E Locascio, J. Hickman, S. Sundaram
Covalent surface modification techniques, in particular surface oxidation procedures, have been employed as a mean to modify polymer microfluidic channels for the purpose of modulating microflow. The focus of this work is to experimentally and comput ...
86. Simultaneous Concentration and Separation of Coumarins Using a Molecular Micelle in Micellar Affinity Gradient Focusing
Mary W Kamande, David J Ross, Laurie E Locascio, Mark Lowry, Isiah M Warner
We report the use of a molecular micelle for the simultaneous separation and concentration of neutral and hydrophobic analytes using micellar affinity gradient focusing (MAGF). The technique, MAGF, combines the favorable features of micellar electrok ...
87. Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
Justin M Zook, Robert I. MacCuspie, Laurie E Locascio, Michael W Halter, John T Elliott
Methods to disperse nanoparticles reproducibly in aqueous solutions compatible with cell-based assays are critical for studying cytotoxicity. Here, we disperse gold and silver nanoparticles in cell culture media, letting them agglomerate to a variet ...
88. Surface Characterization of Laser- Ablated Polymers Used for Microfluidics
D L Pugmire, E A. Waddell, R Haasch, Michael J Tarlov, Laurie E Locascio
Fabrication of microfluidic devices by excimer laser ablation under different atmospheres may provide variations in polymer microchannel surface characteristics. The surface chemistry and electroosmotic (EO) mobility of polymer microchannels laser a ...
89. Surface Modification of PMMA for Application of Wall Coating Polymers for Microchip Electrophoresis
Jayna J Shah, Jon C Geist, Laurie E Locascio, Michael Gaitan, Rao Mulpuri, Wyatt N Vreeland
The development of rapid and simple wall coating strategies for high resolution electrophoretic separation of DNA is of crucial importance for the successful implementation of miniaturized polymeric DNA analysis systems. In this report, we present an ...
90. T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements
Jason G Kralj, Audrey Player, Hope Sedrick, Matthew S Munson, David Petersen, Samuel P Forry, Paul Meltzer, Ernest Kawasaki, Laurie E Locascio
We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microa ...