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Author: margaret kline

Displaying records 11 to 20 of 43 records.
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11. Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples
Published: 1/25/2008
Authors: Carolyn R Hill, Margaret C Kline, Michael Coble, John M Butler
Abstract: An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized to aid in the analysis of degraded DNA samples. These new markers produce short PCR products in the target range of 50 150 base pairs (bp) by movi ...

12. Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits
Published: 7/25/2007
Authors: Carolyn R Hill, Margaret C Kline, Julio J Mulero, Robert E Lagace, Chien-Wei Chang, Lori K Hennessy, John M Butler
Abstract: The AmpFlSTR MiniFiler PCR Amplification kit developed by Applied Biosystems enables size reduction on eight of the larger short tandem repeat (STR) loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded D ...

13. Setting standards and developing technology to aid the human identity testing community
Published: 4/1/2006
Authors: John M Butler, Michael D Coble, Amy E. Decker, David Lee Duewer, Carolyn R Hill, Margaret C Kline, Janette W. Redman, Peter M Vallone
Abstract: Our project team at the U.S. National Institute of Standards and Technology (NIST) is funded by the National Institute of Justice (NIJ) to conduct research that benefits the human identity testing community and to create tools that enable forensic DN ...

14. Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples
Published: 1/27/2006
Authors: John M Butler, Amy E. Decker, Peter M Vallone, Margaret C Kline

15. Testing Candidate DNA Quantitation Standards with Several Real-Time Quantitative PCR Methods
Published: 9/29/2005
Authors: Margaret C Kline, Peter M Vallone, Amy E. Decker, Janette W. Redman, David Lee Duewer, John M Butler

16. Chromosomal Duplications Along the Y-Chromosome and Their Potential Impact on Y-STR Interpretation
Published: 7/1/2005
Authors: John M Butler, Amy E. Decker, Margaret C Kline, Peter M Vallone
Abstract: Y-chromosome short tandem repeat (Y-STR) markers are being touted as potential tools for distinguishing low levels of male DNA in the presence of excess female DNA as is present in many sexual assault samples. Usually single copy Y-STR loci produce a ...

17. Results From the NIST 2004 DNA Quantitation Study
Published: 5/1/2005
Authors: Margaret C Kline, David Lee Duewer, Janette W. Redman, John M Butler
Abstract: For optimal DNA Short Tandem Repeat (STR) typing results, the DNA concentration ([DNA]) of the sample must be accurately determined prior to the polymerase chain reaction (PCR) amplification step in the typing process. In early 2004, the National In ...

18. Mitochondrial DNA Typing Screens With Control Region and Coding Region SNPs
Published: 3/1/2005
Authors: Margaret C Kline, Peter M Vallone, Janette W. Redman, David Lee Duewer, C D Calloway, John M Butler
Abstract: Mitochondrial DNA (mtDNA) analysis has found an important niche in forensic DNA typing. It is used with highly degraded samples or low-copy number materials such as might be found from shed hair or bones exposed to severe environmental conditions. ...

19. NIST Mixed Stain Study 3: Signal Intensity Balance in Commercial Short Tandem Repeat Multiplexes
Published: 12/1/2004
Authors: David Lee Duewer, Margaret C Kline, Janette W. Redman, John M Butler

20. High-Throughput Y-STR Typing of U.S. Populations With 27 Regions of the Y Chromosome Using Two Multiplex PCR Assays
Published: 1/28/2004
Authors: R Schoske, Peter M Vallone, Margaret C Kline, Janette W. Redman, John M Butler
Abstract: Two Y-chromosome short tandem repeat (STR) multiplex polymerase chain reaction (PCR) assays were used to generate haplotypes for 19 single copy and 3 multi-copy Y-STRs. A total of 27 PCR products were examined in each sample using the following loci ...

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