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Author: john kasianowicz

Displaying records 31 to 40 of 62 records.
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31. Conductance and ion selectivity of a mesoscopic protein nanopore probed with cysteine scanning mutagenesis
Published: 11/1/2005
Authors: Petr Merzlyak, Maria-Fatima P. Capistrano, Angela Valeva, John J Kasianowicz, Oleg V Krasilnikov
Abstract: Nanometer-scale, proteinaceous pores are the basis of ion and macromolecular transport in cells and organelles. Recent studies suggest that ion channels and synthetic nanopores may prove useful in biotechnological applications. To better understand t ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=32032

32. Transport of DNA through single nanometer-scale pore: evolution of signal structure
Published: 7/10/2005
Authors: Vincent M Stanford, John J Kasianowicz
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903193

33. Functional Reconstitution of Ion Channels in POlymerizable Lipid Membranes
Published: 6/1/2005
Authors: Devanand K. Shenoy, W Barger, A Singh, Rekha Panchal, M Misakian, Vincent M Stanford, John J Kasianowicz
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=32523

34. Functional Reconstitution of Protein Ion Channels Into Planar Polymerizable Phospholipid Membranes
Published: 6/1/2005
Authors: D K Shenoy, W R Barger, A Singh, R G Panchal, Martin Misakian, Vincent M Stanford, John J Kasianowicz
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830475

35. Purified Bacillus Anthracis Lethal Toxin Complex Formed In Vitro and During Infection Exhibits Functional and Biological Activity
Published: 3/18/2005
Authors: Rekha Panchal, Kelly Halverson, Wilson Ribot, Douglas Lane, Tara Kenny, Teresa G Abshire, John W Ezzell, Timothy A Hoover, Bradford Powell, Stephen Little, John J Kasianowicz, Sina Bavari
Abstract: Anthrax protective antigen (PA, 83 kDa), a pore-forming protein upon protease activation to 63 kDa (PA63), translocates lethal factor (LF) and edema factor (EF) from endosomes into the cytosol of the cell. The relatively small size of the heptameric ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=31879

36. Micro-Electroplating Silver on Sharp Edges for Fabrication of Solid-State Nanopores
Published: 8/11/2004
Authors: Brian J. Polk, Melanie Bernard, John J Kasianowicz, Martin Misakian, Michael Gaitan
Abstract: Electrodeposition of silver was investigated as a fabrication tool for infilling large (103 mm2) vias in silicon substrates. Silver reduction from ammoniacal silver nitrate was studied at electrodes of novel geometry, i.e. the edge of the vias, with ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=31546

37. Membrane Surface Dynamics of DNA-Threaded Nanopores Revealed by Simultaneous Single-Molecule Optical and Ensemble Electrical Recording
Published: 2/3/2004
Authors: E L Chandler, A L Smith, Lisa K Burden, John J Kasianowicz, D L Burden
Abstract: We demonstrate a method for simultaneous real-time electrical and single-molecule optical recordings of the interactions between single-stranded DNA and nanoscopic pores in planar lipid membranes. Electrophysiological techniques are used to measure ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830405

38. Electrostatic Influence on Ion Transport through the alpha HL Channel
Published: 10/1/2003
Authors: M Misakian, John J Kasianowicz
Abstract: The current-voltage relationship of a single Staphylococcus aureus {alpha}-hemolysin({alpha}HL) channel is nonlinear, rectifying, and depends on the bulk pH and the ionic strength. The data are described qualitatively by a simple one-dimensional Ner ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830367

39. Phase Transitions Within the Isolated Polymer Molecule: Coupling of the Polymer Threading a Membrane Transition to the Helix-Random Coil, the Collapse, the Adsorption, and the Equilibrium Polymerization Transitions
Published: 9/22/2003
Authors: Edmund Armando DiMarzio, John J Kasianowicz
Abstract: The polymer threading a membrane transition (PTM), which is a first-order thermodynamic phase transition for an isolated linear polymer in the limit of infinite molecular weight, is coupled to the other 4 phase transitions of the isolated polymer mol ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830408

40. Phase Transitions of a Polymer Threading a Membrane: Character of the Transition When the Molecule can Undergo a Helix-Random Coil or an Equilibrium Polymerization Transition
Published: 1/1/2003
Authors: Edmund Armando DiMarzio, John J Kasianowicz
Abstract: The polymer threading a membrane transition (PTM) which is a first-order thermodynamic phase transition for an isolated linear polymer in the limit of infinite molecular weight is coupled to the helix to random coil (HRC) transition and to the polyme ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830378



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