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Author: marcia holden
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1. A Study of Comparability in AFLP Profiling Using a Simple Model System
Published: 8/15/2007
Authors: Lina Partis, M J Burns, Koichi Chiba, Ph Corbisier, Marcia J Holden, J. Wang, Qing Yan Liu, Tomoya Okunishi, Sang-Ryoul Park, Maxim Vonsky, K R Emslie
Abstract: A simple AFLP model, using the relatively small bacteriophage lambda genome, was developed to test the reproducibility of this technique in an international study, CCQM-P53. Using either non-selective or selective primers, 9 fragments or a subset of ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830545

2. A Thermodynamic Investigation of Some Reactions Involving Prephenic Acid
Published: 2/1/1999
Authors: N Kishore, Marcia J Holden, Yadu D. Tewari, Robert Nathan Goldberg
Abstract: Calorimetric enthalpies of reaction have been measured for the following enzyme-catalyzed reactions at the temperature 298.15 Kprephenate(aq) = phenylpyruvate(aq) + H^d2^0(l) + carbon dioxide(aq), prephenate(aq) + NAD^dox^(aq) = 4-hydroxyphenylpyruva ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830048

3. A Thermodynamic and Quantum Chemical Study of the Conversion of Chorismate to (Pyruvate + 4-Hydroxybenzoate)
Published: 10/22/1998
Authors: Yadu D. Tewari, J Chen, Marcia J Holden, K. N. Houk, Robert Nathan Goldberg
Abstract: A thermodynamic investigation of the conversion of chorismate^u2-^(aq) to {pyruvate^u-^(aq) + 4-hydroxybenzoate^u-^(aq)} has been performed by using microcalorimetry and high-performance liquid chromatography. The study used a genetically engineered ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830045

4. An Inter-Platform Repeatability Study Investigating Real-Time Amplification of Plasmid DNA
Published: 5/25/2005
Authors: C Donald, F Qureshi, M J Burns, Marcia J Holden, J L. Blasic, A J Woolford
Abstract: Background: Currently, there are many different real-time amplification platforms. However, the results of DNA measurements from experiments performed on different platforms are not necessarily comparable and results may not be repeatable within inst ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830446

5. Association and Redox Properties of the Putidaredoxin Reductase - Oxidized Nicotinamide Adenine Dinucleotide Complex
Published: 11/13/2007
Authors: Vytautas Reipa, Marcia J Holden, V L. Vilker
Abstract: Putidaredoxin reductase (PdR) is the flavin protein that carries out the first electron transfer involved in the cytochrome P450cam catalytic cycle. In PdR, the oxidized flavin adenine dinucleotide (FAD/FADH2) redox center is reduced by two-electron ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830517

6. Benzocycloarene Hydroxylation by P450 Biocatalysis
Published: 1/1/2002
Authors: M P. Mayhew, A. E. Roitberg, Yadu D. Tewari, Marcia J Holden, David J Vanderah, V L. Vilker
Abstract: Experimental and theorectical studies of the hydroxylation of a family of benzocycloarene compounds (benzocyclobutene, benzocyclopentene (indan), benzocyclohexene (tetralin), and benzocycloheptene) by wild type P450cam and mutant Y96F P450cam were pe ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830255

7. Binding and Electron Transfer Between Putidaredoxin and Cytochrome P450cam (CYP101). Theory and Experiments
Published: 9/9/1998
Authors: A. E. Roitberg, Marcia J Holden, M P. Mayhew, I V Kurnikov, D N Beratan, V L. Vilker
Abstract: A necessary step in understanding biological function in multiple metalloprotein redox systems is the description of protein-protein binding and electron transfer between two distant metal centers. In this communication, we present experimental and ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830030

8. Challenges in Capturing Oxygenase Activity In Vitro
Published: 11/1/1999
Authors: V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J Holden
Abstract: Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large clas ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830098

9. Chorismate Lyase: Kinetics and Engineering for Stability
Published: 1/31/2002
Authors: Marcia J Holden, M P. Mayhew, David Travis Gallagher, V L. Vilker
Abstract: By removing the enolpyruvyl group from chorismate, chorismate lyase (CL) produces p-hydroxybenzoate (p-HB) for the ubiquinone biosynthetic pathway. We have analyzed CL by several spectroscopic and chemical techniques and measured its kinetic (k^dcat^ ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830209

10. Comparison of Backbone Dynamics of Oxidized and Reduced Putidaredoxin by ^u15^N NMR Relaxation Measurements
Published: 8/3/1999
Authors: Fahriye Nese Sari, Marcia J Holden, M P. Mayhew, V L. Vilker, B Coxon
Abstract: The backbone dynamics of uniformly ^u15^N-labeled reduced and oxidized putidaredoxin (Pdx) have been studied by 2D ^u15^N NMR relaxation measurements. ^u15^N T^d1^ and T^d2^ values and ^u1^H-^u15^N NOE s have been measured for the diamagnetic region ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830085



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