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1. A Study of Comparability in AFLP Profiling Using a Simple Model System
Published: 8/15/2007
Authors: Lina Partis, M J Burns, Koichi Chiba, Ph Corbisier, Marcia J Holden, J. Wang, Qing Yan Liu, Tomoya Okunishi, Sang-Ryoul Park, Maxim Vonsky, K R Emslie
Abstract: A simple AFLP model, using the relatively small bacteriophage lambda genome, was developed to test the reproducibility of this technique in an international study, CCQM-P53. Using either non-selective or selective primers, 9 fragments or a subset of ...

2. A Thermodynamic Investigation of Some Reactions Involving Prephenic Acid
Published: 2/1/1999
Authors: N Kishore, Marcia J Holden, Yadu D. Tewari, Robert Nathan Goldberg
Abstract: Calorimetric enthalpies of reaction have been measured for the following enzyme-catalyzed reactions at the temperature 298.15 Kprephenate(aq) = phenylpyruvate(aq) + H^d2^0(l) + carbon dioxide(aq), prephenate(aq) + NAD^dox^(aq) = 4-hydroxyphenylpyruva ...

3. A Thermodynamic and Quantum Chemical Study of the Conversion of Chorismate to (Pyruvate + 4-Hydroxybenzoate)
Published: 10/22/1998
Authors: Yadu D. Tewari, J Chen, Marcia J Holden, K. N. Houk, Robert Nathan Goldberg
Abstract: A thermodynamic investigation of the conversion of chorismate^u2-^(aq) to {pyruvate^u-^(aq) + 4-hydroxybenzoate^u-^(aq)} has been performed by using microcalorimetry and high-performance liquid chromatography. The study used a genetically engineered ...

4. An Inter-Platform Repeatability Study Investigating Real-Time Amplification of Plasmid DNA
Published: 5/25/2005
Authors: C Donald, F Qureshi, M J Burns, Marcia J Holden, J L. Blasic, A J Woolford
Abstract: Background: Currently, there are many different real-time amplification platforms. However, the results of DNA measurements from experiments performed on different platforms are not necessarily comparable and results may not be repeatable within inst ...

5. Association and Redox Properties of the Putidaredoxin Reductase - Oxidized Nicotinamide Adenine Dinucleotide Complex
Published: 11/13/2007
Authors: Vytautas Reipa, Marcia J Holden, V L. Vilker
Abstract: Putidaredoxin reductase (PdR) is the flavin protein that carries out the first electron transfer involved in the cytochrome P450cam catalytic cycle. In PdR, the oxidized flavin adenine dinucleotide (FAD/FADH2) redox center is reduced by two-electron ...

6. Benzocycloarene Hydroxylation by P450 Biocatalysis
Published: 1/1/2002
Authors: M P. Mayhew, A. E. Roitberg, Yadu D. Tewari, Marcia J Holden, David J. Vanderah, V L. Vilker
Abstract: Experimental and theorectical studies of the hydroxylation of a family of benzocycloarene compounds (benzocyclobutene, benzocyclopentene (indan), benzocyclohexene (tetralin), and benzocycloheptene) by wild type P450cam and mutant Y96F P450cam were pe ...

7. Binding and Electron Transfer Between Putidaredoxin and Cytochrome P450cam (CYP101). Theory and Experiments
Published: 9/9/1998
Authors: A. E. Roitberg, Marcia J Holden, M P. Mayhew, I V Kurnikov, D N Beratan, V L. Vilker
Abstract: A necessary step in understanding biological function in multiple metalloprotein redox systems is the description of protein-protein binding and electron transfer between two distant metal centers. In this communication, we present experimental and ...

8. Challenges in Capturing Oxygenase Activity In Vitro
Published: 11/1/1999
Authors: V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J Holden
Abstract: Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large clas ...

9. Chorismate Lyase: Kinetics and Engineering for Stability
Published: 1/31/2002
Authors: Marcia J Holden, M P. Mayhew, David Travis Gallagher, V L. Vilker
Abstract: By removing the enolpyruvyl group from chorismate, chorismate lyase (CL) produces p-hydroxybenzoate (p-HB) for the ubiquinone biosynthetic pathway. We have analyzed CL by several spectroscopic and chemical techniques and measured its kinetic (k^dcat^ ...

10. Comparison of Backbone Dynamics of Oxidized and Reduced Putidaredoxin by ^u15^N NMR Relaxation Measurements
Published: 8/3/1999
Authors: Fahriye Nese Sari, Marcia J Holden, M P. Mayhew, V L. Vilker, B Coxon
Abstract: The backbone dynamics of uniformly ^u15^N-labeled reduced and oxidized putidaredoxin (Pdx) have been studied by 2D ^u15^N NMR relaxation measurements. ^u15^N T^d1^ and T^d2^ values and ^u1^H-^u15^N NOE s have been measured for the diamagnetic region ...

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