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Additional Technical Details:
For the renewal material SRM 1577c, liver tissue had been collected from 31 steers that were slaughtered at Texas A&M University School of Veterinary Medicine. Each liver was rinsed with HPLC-grade water to remove excess blood, bile, and any other extraneous material. Fresh tissue (120 kg) was harvested from this process, frozen in clean Teflon bags, and shipped to NIST. The tissue was homogenized at NIST with a food processor equipped with titanium blades. The resulting paste was poured into glass trays, frozen, and lyophilized. The dry material was blended in a food processor and then jet-milled. The resulting final product was radiation sterilized, and bottled in 20 g units.
For elemental certification, NIST values for Cd and Ni in serum and Cd, Pb, Se, and Zn in liver were obtained with isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS); all other elements were determined with at least one method carried out at NIST: instrumental (INAA) and radiochemical (RNAA) neutron activation analysis, NAA with pre-concentration of selected elements on Chelex 100 columns (PNAA), ICP-MS, and inductively coupled plasma optical emission spectrometry (ICP-OES). The measurements were complemented by collaborating scientists from research and clinical laboratories using graphite furnace atomic absorption spectrometry (GFAAS), ID gas chromatography (GC)-MS, ICP-MS, ICP-OES, INAA, and RNAA. The CDC priority elements for which certified values are provided are Al, Cr, Cu, Mn, Ni, Se, V, and Zn.
Start Date:October 1, 2002
End Date:October 2008
Lead Organizational Unit:mml
Steven J. Christopher
Related Programs and Projects:
Measurements and Standards for Contaminants in Biological (Human) Materials
Zeisler R., Mackey, E. A., Lamaze, G. P., Stover, T. E., Spatz, R. O., and Greenberg, R. R., “NAA methods for determination of nanogram amounts of arsenic in biological samples,” J. Radioanal. Nucl. Chem., 2006, 269, 291-296
Lindstrom, R.M., Zeisler, R., Greenberg, R.R., “Accuracy and Uncertainty in Radioactivity Measurement for NAA,” J. Radioanal. Nucl. Chem., 2007, 271, 311-315
B.E. Tomlin, R. Zeisler, R.M. Lindstrom, “γ-γ Coincidence Spectrometer for INAA”, Nucl. Instr. Meth., A 589 (2008) 243-249
R. Zeisler, W. D. James, E. A. Mackey, R. O. Spatz, R. R. Greenberg, “NAA Characterization of the New Bovine Liver SRM,” J. Radioanal. Nucl. Chem., 2008, 278, 000-000.
R. Zeisler, R. R. Greenberg, E. A. Mackey, K. E. Murphy, R. O. Spatz, B. E. Tomlin, “Challenges and success in the use of neutron activation analysis procedures for value assignment of anaimal serum and bovine liver Standard Reference Materials,” J. Radioanal. Nucl. Chem., in press.