Luminescence measurements have become the methods of choice for new clinical and biochemical analyses due to their high selectivity and sensitivity. At the same time, the use of Raman measurements for chemical identification, e.g. by first responders, has also increased dramatically. These new methods are becoming increasingly quantitative and require standards to calibrate instruments and to validate the analytical methods themselves. The attendees of recent NIST workshops as well as standards, guidelines and regulatory organizations (e.g. ASTM, USP and FDA) have expressed a need for instrument calibration and performance validation standards in these two key areas of analytical spectroscopy. To work towards meeting this demand, we have developed several novel Raman and fluorescence SRMs.
The Raman-based detection and identification of bio-agents and other chemical species depends on the measurement of relative intensities of Raman signal peaks with varying Raman shift. Similarly, the accuracy of fluorescence-based, quantitative clinical and biochemical assays depends on the measurement of relative intensities of fluorescence with wavelength. Both fluorescence and Raman spectroscopy yield absolute signals, i.e., they are not ratios like absorbance measurements. This means that every instrument has a unique spectral responsivity making both the spectral shape and absolute intensity of a single sample different on every instrument and even on a single instrument at different times. For all of these reasons, relative intensity standards for fluorescence and Raman instrument calibration are needed to obtain accurate and reproducible results.
Our targeted customer base is instrument manufacturers who will use these standards to calibrate their instruments both at the factory and in the field. Stakeholders also include users of these instruments. This type of instrument qualification should aid clinical, pharmaceutical, and other highly regulated industries in satisfying the quality assurance and validation requirements of national health certifiers and regulators, in addition to satisfying their own internal quality assurance standards. This type of standard is also ideal for calibrating portable instruments in the field where conventional calibration techniques are very difficult or impossible to implement.
Produce two suites of SRMs certified for relative intensity, one with a fluorescence emission wavelength range covering the near infrared (NIR), visible and near UV regions of the light spectrum, and the other covering Raman excitation wavelengths of 488 nm, 514.5 nm, 532 nm, 633 nm, 785 nm and 1064 nm with Raman shifts between 150 cm-1 and 3500 cm-1
Research Activities and Technical Approach
DeRose, P.C.; Smith, M.V.; Mielenz, K.D.; Blackburn, D.H. and Kramer, G.W., Characterization of Standard Reference Materials 2940, Mn-Ion-Doped Glass, Spectral Correction Standard for Fluorescence. J. Luminescence 2009, 129, 349-355.
DeRose, P.C.; Smith, M.V.; Mielenz, K.D.; Blackburn, D.H. and Kramer, G.W., Characterization of Standard Reference Materials 2941, Uranyl-Ion-Doped Glass, Spectral Correction Standard for Fluorescence. J. Luminescence 2008, 128, 257-266
DeRose, P.C.; Early, E.A.; Kramer, G.W., Qualification of a Fluorescence Spectrometer for Measuring True Fluorescence Spectra. Rev. Sci. Instru. 2007, 78, 033107.
DeRose, P.C.; Early, E.A.; Kramer, G.W., Measuring and Certifying True Fluorescence Spectra with a Qualified Fluorescence Spectrometer. In: Proceedings of the 5th Oxford Conference on Spectrometry (Crown, UK, 2008).
Start Date:July 1, 2004
Lead Organizational Unit:mml
High Accuracy Fluorescence and Raman Spectrometers