Take a sneak peek at the new NIST.gov and let us know what you think!
(Please note: some content may not be complete on the beta site.).
NIST Authors in Bold
|Author(s):||Li Liu; Daniel W. Bearden; Juan C. Rodriguez; Kathleen S. Rein;|
|Title:||Biosynthesis of Athmu, a alpha,gamma-hydroxy-ß-amino acid of pahayokolides A-B|
|Published:||October 02, 2012|
|Abstract:||Pahayokolides A-B are cyanobacteria derived non-ribosomal peptides which exhibit cytotoxicity against a number of cancer cell lines. The biosynthetic origin of the 3-amino-2,5,7,8- tetrahydroxy-10-methylundecanoic acid (Athmu) moiety has been investigated using stable isotope incorporation experiments. While α-ketoisocaproic acid (α-KIC), α-hydroxyisocaproic acid (α- HIC) and leucine all serve as precursors to Athmu, the feeding of [1-13C] α-KIC results in more than threefold greater 13C enrichment than the other precursors. This result suggests that α-KIC is the immediate precursor which is selected and activated by the adenylation domain of the loading NRPS module and subsequently reduced in a fashion similar to that of the recently identified pathways for cryptophycins A-B, cereulide and valinomycin.|
|Pages:||pp. 6758 - 6760|
|Keywords:||NMR, Pahayokolide, Biosynthesis|
|Research Areas:||NMR, Organic Analytical Chemistry, Chemistry|
|DOI:||http://dx.doi.org/10.1016/j.tetlet.2012.09.119 (Note: May link to a non-U.S. Government webpage)|