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|Author(s):||Carl G. Simon Jr.; Kaushik Chatterjee; Alison M. Kraigsley; Joachim Kohn; Durgadas Bolikal;|
|Title:||Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D|
|Published:||May 01, 2012|
|Abstract:||Current methods for screening cell-material interactions typically utilize two-dimensional (2D) culture format where cells are cultured on flat surfaces. However, there is a need for combinatorial and high-throughput screening methods to systematically screen cell-biomaterial interactions in three-dimensional (3D) tissue scaffolds for tissue engineering. Previously, we developed a two-syringe pump approach for making 3D scaffold gradients for use in combinatorial screening and these scaffold gradients were made by a salt-leaching method. Herein, we demonstrate that the two-syringe pump approach can also be used to create scaffold gradients using a gas-foaming approach. Macroporous foams prepared by a gas-foaming technique are commonly used for fabrication of tissue engineering scaffolds due to their high interconnectivity and good mechanical properties. Gas-foamed scaffold gradient libraries were fabricated from two biodegradable tyrosine-derived polycarbonates: poly(desaminotyrosyl-tyrosine ethyl ester carbonate) (pDTEc) and poly(desaminotyrosyl-tyrosine octyl ester carbonate) (pDTOc). The composition of the libraries was assessed with FTIR and showed that pDTEc/pDTOc gas-foamed scaffold gradients could be repeatably fabricated. Scanning electron microscopy showed that scaffold morphology was similar between the pDTEc-rich ends and the pDTOc-rich ends of the gradient. These results introduce a method for fabricating gas-foamed polymer scaffold gradients that can be used for combinatorial screening of cell-material interactions in 3D.|
|Citation:||Journal of Biomaterials Science Polymer Edition|
|Pages:||pp. 173 - 182|
|Keywords:||cell-material interactions, combinatorial screening, polymer, scaffold, tissue engineering|
|Research Areas:||Polymers, Combinatorial Methods, Processing, Materials Science, Biomaterials, Biomaterials, Bioscience & Health, Advanced Materials|