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Publication Citation: NIST Reference Material Scaffolds Characterized for Cell Response

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Author(s): Carl G. Simon Jr; Jeff M. Coles;
Title: NIST Reference Material Scaffolds Characterized for Cell Response
Published: April 24, 2011
Abstract: Reference scaffolds characterized for cell response are being developed for use as a standard for biological characterization of new scaffolds. The need for reference scaffolds to serve as a calibration standard between labs has been identified as critical to advancing tissue engineering science [1,2]. The Regenerative Medicine Promotion Act was recently introduced into the U.S. House of Representatives which specifically calls for NIST to develop standards for regenerative medicine products [3]. Previously, NIST deployed reference scaffolds with well-characterized structure and porosity (Fig. 1) [4]. The current effort expands the scope to include cell culture data for morphology, attachment and proliferation. Freeform fabrication (FFF) was chosen to make the reference scaffolds since this technique affords precise control of scaffold structure. Poly(-caprolactone) (PCL) was chosen as the polymer since it is used in biomedical implants and is being investigated for tissue scaffold applications. MC3T3-E1 osteoblasts were used for cell response since scaffolds are frequently used for bone tissue engineering. 96-well plate scaffolds were selected for efficiency (fewer reagents, lower cost).
Conference: Society for Biomaterials
Pages: 1 pp.
Location: Orlando, FL
Dates: April 13-16, 2011
Keywords: reference material; scaffold; polymer; tissue engineering; cell proliferation; cell adhesion; freeform fabricated scaffold
Research Areas: Polymers, Polymers, Materials Science, Biomaterials, Biomaterials, Biomaterials, Bioscience & Health, Advanced Materials