| Author(s): |
Kate L. Klein; Ian M. Anderson; N. de Jonge; |
| Title: |
Tranmission Electron Microscopy with a Liquid Flow Cell |
| Published: |
January 20, 2011 |
| Abstract: |
The imaging of microscopic structures at nanometer-scale spatial resolution in a liquid environment is of interest for a wide range of studies. Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness. |
| Citation: |
Journal of Microscopy |
| Volume: |
242 |
| Pages: |
pp. 117 - 123 |
| Keywords: |
in situ; liquid; nanoparticles; STEM; TEM |
| Research Areas: |
Nanotechnology |
| PDF version: |
 Click here to retrieve PDF version of paper (573KB) |
