Klein, K.
, Anderson, I.
and de, N.
(2011),
Tranmission Electron Microscopy with a Liquid Flow Cell, Journal of Microscopy, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=906875
(Accessed October 22, 2025)
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Tranmission Electron Microscopy with a Liquid Flow Cell
Published
Author(s)
, , N. de Jonge
Abstract
The imaging of microscopic structures at nanometer-scale spatial resolution in a liquid environment is of interest for a wide range of studies. Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness.Citation
Journal of Microscopy
Volume
242
Pub Type
Journals
Download Paper
Keywords
in situ, liquid, nanoparticles, STEM, TEM
Citation
Issues
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Created January 20, 2011, Updated February 19, 2017