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Publication Citation: Tranmission Electron Microscopy with a Liquid Flow Cell

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Author(s): Kate L. Klein; Ian M. Anderson; N. de Jonge;
Title: Tranmission Electron Microscopy with a Liquid Flow Cell
Published: January 20, 2011
Abstract: The imaging of microscopic structures at nanometer-scale spatial resolution in a liquid environment is of interest for a wide range of studies. Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness.
Citation: Journal of Microscopy
Volume: 242
Pages: pp. 117 - 123
Keywords: in situ, liquid, nanoparticles, STEM, TEM
Research Areas: Nanotechnology
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