NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Tranmission Electron Microscopy with a Liquid Flow Cell

Published

Author(s)

Kate L. Klein, Ian M. Anderson, N. de Jonge

Abstract

The imaging of microscopic structures at nanometer-scale spatial resolution in a liquid environment is of interest for a wide range of studies. Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness.
Citation
Journal of Microscopy
Volume
242
Pub Type
Journals

Download Paper

Local Download

Keywords

in situ, liquid, nanoparticles, STEM, TEM
Nanotechnology

Citation

Klein, K. , Anderson, I. and de, N. (2011), Tranmission Electron Microscopy with a Liquid Flow Cell, Journal of Microscopy, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=906875 (Accessed October 11, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created January 20, 2011, Updated February 19, 2017
Was this page helpful?