Take a sneak peek at the new NIST.gov and let us know what you think!
(Please note: some content may not be complete on the beta site.).

View the beta site
NIST logo

Publication Citation: STR Sequence Analysis for Characterizing Normal, Variant, and Null Alleles

NIST Authors in Bold

Author(s): Margaret C. Kline; Carolyn R. Steffen; John M. Butler; Amy Decker;
Title: STR Sequence Analysis for Characterizing Normal, Variant, and Null Alleles
Published: August 01, 2011
Abstract: DNA sequence variation is known to exist in and around the repeat region of short tandem repeat (STR) loci used in human identity testing. While the vast majority of STR alleles measured in forensic DNA laboratories worldwide type as ,normalŠ alleles compared with STR kit allelic ladders, a number of variant alleles have been reported. In addition, a sequence difference at a polymerase chain reaction (PCR) primer binding site in the DNA template can cause allele drop-out (i.e., a ,nullŠ or ,silentŠ allele) with one set of primers and not with another. Our group at the National Institute of Standards and Technology (NIST) has been sequencing variant and null alleles supplied by forensic labs and cataloging this information on the NIST STRBase website for the past decade. The PCR primer sequences and strategy used for our STR allele sequencing work involving 23 autosomal STRs and 17 Y-chromosome STRs are described along with the results from 113 variant and 16 null alleles.
Citation: Forensic Science International: Genetics
Volume: 5
Issue: 4
Pages: pp. 329 - 332
Keywords: short tandem repeat, STR typing, DNA sequencing, allele dropout, null allele, variant allele
Research Areas: Forensics, Chemistry
PDF version: PDF Document Click here to retrieve PDF version of paper (213KB)