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Publication Citation: Low-Cost, High-Throughput, Automated Counting of Bacterial Colonies

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Author(s): Matthew L. Clarke; Robert L. Burton; A. N. Hill; Maritoni A. Litorja; Moon H. Nahm; Jeeseong Hwang;
Title: Low-Cost, High-Throughput, Automated Counting of Bacterial Colonies
Published: January 05, 2010
Abstract: Research involving bacterial pathogens often requires enumeration of bacteria colonies. Here we present a low-cost, high-throughput colony counting system consisting of colony counting software and a consumer-grade digital camera or document scanner. We demonstrate that this software, called NICE (NIST s Integrated Colony Enumerator), can count bacterial colonies as part of a high-throughput multiplexed opsonophagocytic killing assay (MOPA), which is used to characterize pneumococcal vaccine efficacy. The results obtained with NICE correlate well with the results obtained from manual counting, with a mean difference of less than 3%. NICE is also rapid; it can count colonies from multiple reaction wells within minutes and export the results to a spreadsheet for data processing. As this program is freely available from NIST, NICE should be helpful in bacteria colony enumeration required in many microbiological studies, and in standardizing colony counting methods.
Citation: Cytometry Part A
Volume: 77A
Issue: 8
Pages: pp. 790 - 797
Keywords: bacteria; colony counting; imaging; MOPA; pneumoniae; software; vaccine
Research Areas: Optical Metrology, Biological Product Characterization, Imaging
PDF version: PDF Document Click here to retrieve PDF version of paper (976KB)