| Author(s): |
Dustin Levy; Kimberly A. Briggman; |
| Title: |
Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes |
| Published: |
May 25, 2007 |
| Abstract: |
The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder transitions of the lipid acyl chains was used to determine the main gel to liquid crystal phase transition temperature, Tm, for HBMs of binary cholesterol/phospholipid mixtures on octadecanethiolate self-assembled monolayers. The phase transition temperature and the breadth of the transition were shown to increase with cholesterol content, and the phase boundaries observed in the cholesterol/phospholipid HBMs were comparable to the published phase diagrams of binary cholesterol/phospholipid vesicles. A thermodynamic assessment of the cooperative units of theHBMphase transitions revealed the presence of<10nmdiameter domains that were independent of the cholesterol composition. |
| Citation: |
Biophysical Journal |
| Volume: |
23 |
| Pages: |
pp. 7155 - 7161 |
| Keywords: |
cholesterol-lipid phase diagrams;hybrid bilayer membranes;SFG;supported bilayer membrane mimics;vibrational sum frequency |
| Research Areas: |
Biological Physics, Nonlinear Optical Spectroscopy, Bioscience & Health, Optical Technology |
| PDF version: |
 Click here to retrieve PDF version of paper (126KB) |
