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|Author(s):||Dustin Levy; Kimberly A. Briggman;|
|Title:||Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes|
|Published:||May 25, 2007|
|Abstract:||The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder transitions of the lipid acyl chains was used to determine the main gel to liquid crystal phase transition temperature, Tm, for HBMs of binary cholesterol/phospholipid mixtures on octadecanethiolate self-assembled monolayers. The phase transition temperature and the breadth of the transition were shown to increase with cholesterol content, and the phase boundaries observed in the cholesterol/phospholipid HBMs were comparable to the published phase diagrams of binary cholesterol/phospholipid vesicles. A thermodynamic assessment of the cooperative units of theHBMphase transitions revealed the presence of<10nmdiameter domains that were independent of the cholesterol composition.|
|Pages:||pp. 7155 - 7161|
|Keywords:||cholesterol-lipid phase diagrams,hybrid bilayer membranes,SFG,supported bilayer membrane mimics,vibrational sum frequency|
|Research Areas:||Biological Physics, Nonlinear Optical Spectroscopy, Bioscience & Health, Optical Technology|
|PDF version:||Click here to retrieve PDF version of paper (130KB)|