The Development of Fluorescence Intensity Standards

Published

March 1, 2001

Author(s)

Adolfas K. Gaigalas, L. Li, O. Henderson, R F. Vogt, J. Barr, G E. Marti, J. Weaver, A. Schwartz

Abstract

The use of fluorescence as an analytical technique has been growing over the last 20 years. A major factor in inhibiting more rapid growth has been the inability to make comparable fluorescence intensity measurements across laboratories. NIST recognizes the need to develop and provide primary fluorescence intensity standard (FIS) reference materials to the scientific and technical communities involved in these assays. The critical component of the effort will be the cooperation between the Federal laboratories, the manufacturers and the technical personnel who will use the fluorescence intensity standards. We realize that the development and use of FIS will have to overcome many difficulties, however, as we outline in the following article, the development of FIS is feasible.

Citation

Journal of Research (NIST JRES) -

Volume

106

Issue

No. 2

NIST Pub Series

Journal of Research (NIST JRES)

Pub Type

NIST Pubs

Keywords

fluorescence intensity, quantitative fluorescence, standards

Citation

Gaigalas, A. , Li, L. , Henderson, O. , Vogt, R. , Barr, J. , Marti, G. , Weaver, J. and Schwartz, A. (2001), The Development of Fluorescence Intensity Standards, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD (Accessed October 18, 2025)