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Publication Citation: 3D Particle Trajectories Observed by Orthogonal Tracking Microscopy

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Author(s): Matthew D. McMahon; Andrew J. Berglund; Peter T. Carmichael; Jabez J. McClelland; James A. Liddle;
Title: 3D Particle Trajectories Observed by Orthogonal Tracking Microscopy
Published: February 09, 2009
Abstract: We demonstrate high-resolution, high-speed 3D nanoparticle tracking using angled micromirrors. When angled micromirrors are introduced into the field of view of an optical microscope, reflected side-on views of a diffusing nanoparticle are projected alongside the usual direct image. The experimental design allows us to find the 3D particle trajectory using fast, centroid-based image processing, with no nonlinear computing operations. We have tracked polystyrene particles of 190 nm diameter with position measurement precision <20 nm in 3D with 3 ms frame duration (i.e., at an imaging rate >330 frames per second). Because the image processing requires only ≈1 ms per frame, this technique could enable real-time feedback-controlled nanoparticle assembly applications with nanometer precision.
Citation: ACS Nano
Volume: 3
Pages: pp. 609 - 614
Keywords: orthogonal tracking microscopy; particle tracking; three-dimensional microscopy; optical microscopy; digital video microscopy; multiple vantage point microscopy
Research Areas: Optical microscopy
PDF version: PDF Document Click here to retrieve PDF version of paper (637KB)