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Chemistry Programs & Projects

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Chromatographic Separation of Unfolded Proteins for Protein Quantification
Last Updated Date: 01/20/2015

NIST is developing methods for the quantification of protein biomarkers by mass spectrometry (MS). Stable isotope labeling of proteins/peptides … more

Development of Reference Methods and Reference Materials for the Determination of Cardiac Troponin I in Human Serum
Last Updated Date: 01/20/2015

Cardiac troponin I (cTnI) is considered the 'gold standard' for the diagnosis of myocardial infarction and the assessment of cardiac damage. Yet … more

Development of Proteomics-Based Methods for Protein Quantification
Last Updated Date: 01/20/2015

NIST is developing methods for the quantification of clinically-relevant protein biomarkers by mass spectrometry. Some methods are intended to … more

Development of a Reference Measurement Procedure for C-Reactive Protein in Human Serum
Last Updated Date: 01/20/2015

C-reactive protein (CRP) levels in serum or plasma have been used as an indicator of inflammation and infection. Recent evidence also suggests … more

Improving Measurement Quality in Clinical Proteomics
Last Updated Date: 01/20/2015

Through collaboration with academic and government institutes, NIST is critically evaluating and attempting to improve measurement quality in … more

Development of an Amino Acid (in Solution) Standard Reference Material
Last Updated Date: 01/20/2015

Amino acids are the sequential components of proteins and many are essential to our physiological well-being. Their quantification by the … more

MEMS Measurement Science and Standards
Last Updated Date: 01/20/2015

Our goal is to provide technical leadership in R&D for the MEMS measurement infrastructure essential to improving U.S. economic … more

Thermodynamics of Enzyme-catalyzed Reactions
Last Updated Date: 01/14/2015

To develop the thermodynamic data and methodology required for effective bioprocess engineering.

Standards for Immuno-Histochemical Imaging: A Biomarker Protein Expression Chip
Last Updated Date: 01/14/2015

Immunohistochemistry (IHC) is used to visualize antigens in tissue sections by specific antigen-antibody interactions, an advantage over … more

Protection Against Ionizing Radiation in Extreme Radiation-Resistant Microorganisms
Last Updated Date: 01/14/2015

We measured the levels of modified DNA bases following ?-irradiation of the halophilic archaeon, Halobacterium sp. str. NRC-1. In this first study … more

Development of Enhanced mRNA Amplification using Microfluidics
Last Updated Date: 01/14/2015

In conjunction with NCI/NIH, NIST is developing methods to improve mRNA amplification from small biological samples. Typical sample processing … more

Fundamental Interaction Mechanisms of Engineered Nanomaterials with DNA
Last Updated Date: 01/14/2015

We utilized isotope-dilution liquid chromatography/mass spectrometry (LC/MS) to determine the levels of the oxidatively damaged DNA bases, … more

Multiplexed Detectorless Electrophoresis
Last Updated Date: 01/14/2015

The ability to acquire large amounts of high quality data very rapidly is increasingly important for progress in the biological sciences. … more

Controlling Microfluidic Cell Culture Environments
Last Updated Date: 01/14/2015

NIST has taken advantage of recent technological advances to develop polymer microfluidic cell culture systems that provide a level of control … more

Dental Materials Project
Last Updated Date: 01/14/2015

Our goal is to enable the development and manufacturing of biomaterials for oral health moving forward in 21st century by providing critical … more

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