Transmembrane protein (TMP)-functionalized analytical platforms represent a powerful new paradigm in chemical analysis. Of particular interest is the development of high throughput, TMP-functionalized stationary phases for affinity chromatography of complex analyte libraries. A number of natural and synthetic phospholipids and lipid-mimics have been utilized for TMP reconstitution, though the resulting membranes often lack the requisite chemical and temporal stability for long term utilization, a problem that is exacerbated in flowing separation systems. Over the past two decades, polymerizable lipids have been developed that exhibit markedly increased membrane stability, while still supporting TMP function. More recently, these lipids have been incorporated into a range of analytical platforms, including separation-based platforms, and are now poised to make significant impacts in TMP-based separations. Here, we describe the current methods for preparing TMP-containing stationary phases and examine the potential utility of polymerizable lipids in TMP affinity chromatography.
Citation: Analytical and Bioanalytical Chemistry
Pub Type: Journals
lipid, stationary phase, chromatography