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Search Publications by: Michael Gaitan (Assoc)

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Displaying 51 - 75 of 164

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Author(s)
Michael Gaitan, Jayna J. Shah, Darwin Reyes-Hernandez, Pierre-Alain Auroux, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Liposome-Templated Supramolecular Assembly of Responsive Alginate Nanogels

March 14, 2008
Author(s)
Jennifer S. Hong, Wyatt N. Vreeland, Silvia H. De Paoli Lacerda, Laurie E. Locascio, Michael Gaitan, Srinivasa R. Raghavan
Nanosized gel particles (nanogels) are of interest for a variety of applications, including controlled release of drugs and single-molecule encapsulation. Here, we employ the cores of nanoscale liposomes as reaction vessels to template the assembly of

Preparation of Nanoparticles by Continuous-Flow Microfluidics

February 15, 2008
Author(s)
Andreas Jahn, Joseph E. Reiner, Wyatt N. Vreeland, Don DeVoe, Laurie E. Locascio, Michael Gaitan
We review a variety of micro- and nanoparticle formulations produced with microfluidic methods. A diverse variety of approaches to generate micro-scale and nano-scale particles have been reported. Here we emphasize the use of microfluidics, specifically

Young's Modulus Measurements in Standard IC CMOS Processes using MEMS Test Structures

November 1, 2007
Author(s)
Janet M. Cassard, David L. Herman, Paul T. Vernier, Don DeVoe, Michael Gaitan
This letter presents a method to measure the Young?s moduli of the individual thin film layers in a commercial integrated circuit (IC) foundry process. The method is based on measuring the resonance frequency of an array of micromachined cantilevers and

Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating

October 15, 2007
Author(s)
Pierre-Alain Auroux, Darwin R. Reyes-Hernandez, Jayna J. Shah, Michael Gaitan
We report 100% yield and 40% reaction-time reduction for on-chip DNA amplification using microwave dielectric heating. A passivation protocol has also been developed to suppress the inhibition of PCR in PMMA chips used with a temperature sensor. We finally

Microwave Dielectric Heating of Fluids in an Integrated Microfluidic Device

October 3, 2007
Author(s)
Jayna J. Shah, Siddarth Sundaresan, Jon Geist, Darwin Reyes-Hernandez, James Booth, Rao Mulpuri, Michael Gaitan
Rapid, selective, and uniform heating of sub-microliter size fluid volumes is vital for micro total analysis system (?gTAS) applications. In this work, we present an approach to localized and selective heating of fluids in a microfluidic network. This is

Microwave Power Absorption in Low-Reflectance, Complex, Lossy Transmission Lines

August 1, 2007
Author(s)
Jon Geist, Jayna J. Shah, Mulpuri Rao, Michael Gaitan
Simple sets of equations have been derived to describe the absorption of microwave power in threeregion, lossy transmission lines in terms of S-parameter reflection and transmission amplitudes. Each region was assumed to be homogeneous with discontinuities

Liposome Characterization with Fluorescence Cumulant Analysis

June 8, 2007
Author(s)
Joseph E. Reiner, Andreas Jahn, Laurie E. Locascio, Michael Gaitan, John J. Kasianowicz
Liposomes are self-assembled spherical vesicles comprised of a lipid bilayer membrane that segregates an internal aqueous environment from an external aqueous environment. These nanometer-scale structures have demonstrated potential for targeted drug

Microfluidic Directed Formation of Liposomes of Controlled Size

April 24, 2007
Author(s)
Andreas Jahn, Wyatt N. Vreeland, Don DeVoe, Laurie E. Locascio, Michael Gaitan
A new method to tailor liposome size and size distribution in a microfluidic format is presented. Liposomes are spherical structures formed from lipid bilayers that are from tens of nanometers to several micrometers in diameter. Liposome size and size

Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating

November 9, 2006
Author(s)
Pierre-Alain Auroux, Jayna J. Shah, James Booth, Mulpuri Rao, Laurie E. Locascio, Michael Gaitan
We report on temperature time domain and spectral measurements for on-chip microwave thermocycling. In particular we observe enhanced specificity of the heating of a targeted sample compared to conventional heating block-based thermocyclers. Spatial

Photopatterned Polyelectrolyte Multilayers for Cell Culture

November 9, 2006
Author(s)
Darwin Reyes-Hernandez, J. Brian Hutchison, Laurie E. Locascio, Michael Gaitan
Patterned two-dimensional cell cultures are increasingly important for constructing bioanalytical tools for cell and molecular biology to address questions about interactions, signaling, migration, and differentiation, among others. This contribution

The Study of the U.S. Measurement System for Micro Nano Technologies

September 1, 2006
Author(s)
Richard A. Allen, Craig McGray, Michael Gaitan
The National Institute of Standards and Technology (NIST) has launched an ambitious assessment of the nation''s decentralized measurement system, that is far more encompassing than the few studies done over the last several decades. The aim is to determine

Capillarity Induced Solvent?Actuated Bonding of Polymeric Microfluidic Devices

April 12, 2006
Author(s)
Jayna J. Shah, Jon C. Geist, Laurie E. Locascio, Michael Gaitan, Rao Mulpuri, Wyatt N. Vreeland
Rapid, robust and economical fabrication of fluidic microchannels is of fundamental importance for the successful development of disposable lab-on-a-chip devices. In this work, we present a solvent-actuated bonding method for fabricating polymeric

Ag/AgCl Microelectrodes with Improved Stability for Microfluidics

March 30, 2006
Author(s)
Brian J. Polk, Anna Stelzenmuller, Geraldine I. Mijares, William A. MacCrehan, Michael Gaitan
A method for fabricating Ag/AgCl planar microelectrodes for microfluidic applications is presented. Microreference electrodes enable accurate measurements with miniaturized chemical sensors but such electrodes often exhibit very limited useable lifetimes