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Displaying 1 - 25 of 1427

Certification of Standard Reference Material(R) 965c Glucose in Frozen Human Serum

March 13, 2025
Author(s)
Amanda Bayless, William Davis, Elena Wood, Abraham Kuri Cruz, Johanna Camara, Blaza Toman, Komal Dahya, Katherine Earl, Chui Tse, Uliana Danilenko, Hubert Vesper
Standard Reference Material (SRM) 965c Glucose in Frozen Human Serum is intended for use in validating methods for the determination of glucose in human serum and plasma. A unit of SRM 965c consists of three ampoules each of four materials: Level 1, Level

Role of NEIL1 in genome maintenance

February 19, 2025
Author(s)
Amanda McCullough, Irina Minko, Michael Luzadder, Jamie Zuckerman, Pawel Jaruga, Vladimir Vartanian, M Miral Dizdar, R. Stephen Lloyd
Phylogenetic analyses of DNA glycosylases that function in the initiation step of base excision repair reveal a high degree of conservation within the genes encoding Nei-like DNA glycosylase 1 (NEIL1). In concert with other glycosylases, this enzyme is an

Impacts of household water demands and water heater delivery temperatures on opportunistic premise plumbing pathogens (OPPPs) in a residential setting

February 1, 2025
Author(s)
Alshae Logan, Jumana Aljafari, Visesh Uppoor, Tania Ullah, Jennifer Dootz
Efforts aimed towards water and energy conservation within residential buildings may create enhanced conditions for Opportunistic Premise Plumbing Pathogens (OPPPs) growth. To investigate this issue, a study was conducted to assess the effect of water

Aflatoxin B1-induced DNA adduct formation in murine kidney and liver

January 31, 2025
Author(s)
Pawel Jaruga, Vladimir Vartanian, Irina Minko, M Miral Dizdar, Amanda McCullough, R. Stephen Lloyd
Aflatoxicosis is a life-threatening nephrotoxic condition arising from eating foods highly contaminated with aflatoxin-producing molds. Additionally, chronic aflatoxin exposures are linked to enhanced hepatocellular carcinomas. Using recent advances in

Measuring the size of oil droplets in a flow cytometer using Mie Resonances

January 21, 2025
Author(s)
Richard E. Cavicchi, Dean Ripple, Joshua Welsh, Jerilyn Izac, Alexander Peterson, Aaron Goldfain, Wyatt N. Vreeland
An emulsion of silicone oil droplets in aqueous buffer produces a distinctive series of peaks or resonances in the side scatter histogram in a flow cytometer. As many as 12 peaks are observed in the violet-side scatter channel at 405 nm, with half that

Commutability Assessment of New Standard Reference Materials (SRMs) for Determining Serum Total 25-Hydroxyvitamin D using Ligand Binding and Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS) Assays

January 10, 2025
Author(s)
Stephen Wise, Etienne Cavalier, Pierre LUKAS, Stephanie Peeters, caroline Le Goff, Laura Briggs, Emma Williams, Ekaterina Mineva, Christine Pfeiffer, Hubert Vesper, Christian Popp, Christian Beckert, Jan Schultess, Kevin Wang, Carole Tourneur, Camille Pease, Dominik Osterritter, Ralf Fischer, Ben Saida, Chao Dou, Satoshi Kojima, Hope Weiler, Agnieszka Bielecki, Heather Pham, Alexandra Bennett, Shawn You, Amit Ghoshal, Chistian Vogl, James Freeman, Neil Parker, Samantha Pagliaro, Jennifer Cheek, Jie Li, Hisao Tsukamoto, Karen Galvin, Kevin Cashman, Hsuan-Chieh Liao, Andrew Norbert Hoofnagle, Jeffery Budd, Adam Kuszak, Ashley Boggs-Russell, Carolyn Burdette, Grace Hahm, Federica Nalin, Johanna Camara
Commutability is where the measurement response for a reference material (RM) is the same as for an individual patient sample with the same concentration of analyte measured using two or more measurement systems. Assessment of commutability is essential

Interlaboratory Measurement of Adeno-Associated Virus: Comparative Quantification of Full and Empty Capsids

December 26, 2024
Author(s)
Sean Lehman, Wyatt N. Vreeland, Jerome Jacques, Sharee Adams-Hall, Shreya Ahuja, Adnan Arnaout, Hunter Balduf, Ivan Budyak, Thomas Cleveland, Dennis Delgado, James Deng, Brandon Doyle, David L. Duewer, Carsten Elger, Jeffrey Fagan, Tim Guo, Jorge Haller, Luisa Hilgenfeld, Van Hoang, Allison Huldin, Matthew Hyatt, Sambit Kar, Sandeep Kedia, Bashkim Kokona, Amy Liu, Li Ma, Diane McCarthy, He Meng, Easton Noble, Veronika Oettle, Andrew Pla, Thomas Powers, Dean Ripple, Herbert Runnels, Raphael Ruppert, Florian Semmelmann, Christopher Sims, Saurabh Singh, Ronald Toth, Austin Vogt, Sabine Wenzel, Neal Whitaker, Zhiwen Yang, Brandon Zhuang
Recombinant adeno-associated virus (AAV) is one of the main viral vector-based gene therapy platforms. AAV is a virus consisting of a ≈25 nm diameter capsid with a ≈4.7 kb cargo capacity. The safety and efficacy depend on the correct encapsidation of the

The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing

December 26, 2024
Author(s)
Samreen Falak, Denise O'Sullivan, Megan Cleveland, Simon Cowen, Eloise Busby, Alison Devonshire, Esmeralda Valiente, Gerwyn Jones, Martin Kammel, Mojca Milavec, Laura Vierbaum, Ingo Schellenberg, Heinz Zeichhardt, Andreas Kummrow, Peter Vallone, Rainer Macdonald, Jim Huggett
Background Nucleic Acid Amplification Tests (NAATs) are used to diagnose many infectious diseases. They are typically sensitive and specific and can be quickly developed and adapted. It is more challenging to develop standards to ensure NAATs perform

Enabling Global Image Data Sharing in the Life Sciences

December 23, 2024
Author(s)
Anne L. Plant, Peter Bajcsy
Public, reference data are one of the most important foundational resources in the modern life and biomedical sciences. Over the last 40 years, the public release and availability of genomic and macromolecule structural databases accelerated discovery

Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation

December 20, 2024
Author(s)
Patricia Kiesler, Samantha Maragh, Stella Lee, Alexis Norris, Mayumi Miller, Adam Moyer
Genome editing technology is being used in animals for a variety of purposes, including improvement of animal and public health outcomes. Characterization of genome editing reagents and anticipated genomic alterations is an essential step toward the

Recommendations for assessing commutability of a replacement batch of a secondary calibrator certified reference material

December 16, 2024
Author(s)
Liesbet Deprez, Jesper Johansen, Thomas Keller, Jeffrey Budd, Neil Greenberg, Cas Weykamp, Sverre Sandberg, MAURO PANTEGHINI, Ferruccio Ceriotti, Elizabeth Barczak, Robert Rej, Pernille Kjeilen Fauskanger, Finlay MacKenzie, Johanna Camara, Alicia Lyle, W. Greg Miller, VINCENT DELATOUR
Commutable secondary certified reference materials (CRMs) play an essential role in the calibration hierarchy of many in-vitro diagnostic measurement procedures used in the medical laboratory. Therefore, sustainable availability of these CRMs is crucial to

Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report

November 20, 2024
Author(s)
Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski
The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and

Results from a multi-laboratory ocean metaproteomic intercomparison: effects of LC-MS acquisition and data analysis procedures

November 8, 2024
Author(s)
Mak Saito, Jaclyn Saunders, Matthew McIlvin, Erin Bertrand, John Breier, Margaret Brisbin, Sophie Colston, Jaimee Compton, Tim Griffin, Judson Harvey, Robert Hettich, Pratik Jagtap, Michael Janech, Rod Johnson, Rick Keil, Hugo Kleikamp, Dasha Leary, Scott McCain, Eli Moore, Subina Mehta, Dawn Moran, Jacqui Neibauer, Benjamin Neely, Michael Jakuba, Jim Johnson, Megan Duffy, Gerhard Herndl, Richard Giannone, Ryan Mueller, Brook Nunn, Martin Pabst, Samantha Peters, Andrew Rajczewski, Elden Rowland, Brian Searle, Tim Van Den Bossche, Gary Vora, Jacob Waldbauer, Haiyan Zheng, Zihao Zhao
Metaproteomics is an increasingly popular methodology that provides information regarding the metabolic functions of specific microbial taxa and has potential for contributing to ocean ecology and biogeochemical studies. A blinded multi-laboratory

Color-map recommendation for MR relaxometry maps

October 16, 2024
Author(s)
Miha Fuderer, Fabio Crameri, Nandita deSouza, Bettina Baessler, Vikas Gulani, Meiyun Wang, Ruud de Boer, Matt Cashmore, Wolter de Graaf, Kathryn Keenan, Dan Ma, Carolin Pirkl, Nico Sollmann, Sebastian Weingartner, Stefano Mandija, Xavier Golay

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

October 3, 2024
Author(s)
Tracey Schock, Christina Jones, Federico Torta, Nils Hoffmann, Bo Burla, Irina Alecu, Makoto Arita, Takeshi Bamba, Steffany A.L. Bennett, Justine BERTRAND-MICHEL, Britta Brugger, Monica Molina, Maria Dolores Camacho Munoz, Antonio Checa, Michael Chen, Michelle Cinel, Benoit COLSCH, Cristina Coman, Bebiana Da Costa Sousa, Alex Dickens, Maria Maria, Finnur Freyr Eiriksson, Hector Gallart-Ayala, Mohan Ghorasaini, Martin Giera, Xue Guan, Mark Haid, Thomas Hankemeier, Amy Harms, Marcus Horing, Michal Holcapek, Thorsten Hornemann, Chunxiu Hu, Andreas Hulsmeier, Julijana Ivanisevic, Harald Kofeler, Sin Man Lam, Jong Cheol Lee, Gerhard Liebisch, Andrea F Lopez-Clavijo, Malena Manzi, Peter Meikle, Maria Monge, Sneha Muralidharan, Anna Nicolaou, Valerie O'Donnell, Matej Oresic, Arvind Ramanathan, Daisuke Saigusa, Heidi Schwartz-Zimmermann, Guanghou Shui, Masatomo Takahashi, Margret Thorsteinsdottir, Anthony Tournadre, Hiroshi Tsugawa, Victoria Tyrrell, Grace van der Gugten, Michael Wakelam, Craig Wheelock, Denise Wolrab, Guowang Xu, John Bowden, Kim Ekroos, Robert Ahrends, Markus Wenk
In this community effort, we compared measurements between 34 laboratories from 19 countries, utilizing mixtures of authentic synthetic standards, to quantify by mass spectrometry four ceramide species in the NIST human blood plasma Standard Reference

Jointly benchmarking small and structural variant calls with vcfdist

October 2, 2024
Author(s)
Tim Dunn, Justin Zook, James Holt, Satish Narayanasamy
In this work, we extend vcfdist to be the first variant call benchmarking tool to jointly evaluate phased single-nucleotide polymorphisms (SNPs), small insertions/deletions (INDELs), and structural variants (SVs) for the whole genome. First, we find that a

Feasibility of Metrological Traceability Implementation Using the Joint Committee on Traceability in Laboratory Medicine Database Entries Including the Fulfillment of "Fit-for-Purpose" Maximum Allowable Measurement Uncertainty

September 24, 2024
Author(s)
MAURO PANTEGHINI, Johanna Camara, VINCENT DELATOUR, Katleen Van Uytfanghe, Hubert Vesper, Tianjiao Zhang
Background: In previous publications, the Task Force on Reference Measurement System Implementation proposed a procedural approach combining a critical review of entries available in the Joint Committee on Traceability in Laboratory Medicine (JCTLM)
Displaying 1 - 25 of 1427