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Displaying 76 - 100 of 233

The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo

March 16, 2010
Minin Liu, Viswanath Bandaru, Jeffrey Bond, Pawel Jaruga, Xiaobei Zhao, Plamen P. Christov, Cynthia Burrows, Carmelo J. Rizzo, Miral M. Dizdar, Susan Wallace
To protect cells from oxidative DNA damage and mutagenesis, organisms possess multiple glycosylases to recognize the damaged bases and to initiate the Base Excision Repair (BER) pathway. Recently, three DNA glycosylases were identified in mammals that are

The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate specificities from their Escherichia coli counterparts

February 4, 2010
Yin Guo, Viswanath Bandaru, Pawel Jaruga, Xiaobei Zhao, Cynthia Burrows, Shigenori Iwai, Miral M. Dizdar, Jeffrey Bond, Susan Wallace
The DNA glycosylases function in the first step of the base excision repair process that is responsible for removing endogenous oxidative purine and pyrimidine damages from DNA. The DNA glycosylases that remove oxidized DNA bases fall into two general

A New 26plex Autosomal STR Assay to Aid Human Identity Testing*(dagger)

September 1, 2009
Carolyn R. Steffen, John M. Butler, Peter Vallone
Multiplex polymerase chain reaction (PCR) has become the standard in forensic testing. Currently there are two commercial multiplex PCR amplification kits available that simultaneously amplify 16 short tandem repeat (STR) loci that include the 13 FBI

Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase

September 1, 2009
Viktoriya Sidorenko, Arthur P. Grollman, Pawel Jaruga, Miral M. Dizdar, Dmitry Zharhov
Human 8-oxoguanine-DNA-glycosylase (OGG1) efficiently removes mutagenic 8-oxoguanine (8-oxoGua) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) when paired with cytosine in damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to GT

Factors Affecting Quantification of Total DNA by UV Spectroscopy and PicoGreen Fluorescence

August 26, 2009
Marcia J. Holden, Ross J. Haynes, Savelas A. Rabb, Neena Satija, Kristina Yang, Joe Blasic
The amount of total DNA in a preparation extracted from tissues can be measured several ways, each method offering advantages and disadvantages. It is of interest for the sake of accuracy in quantitation to compare methodologies and determine if good

Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases

May 1, 2009
Scott D. Kathe, Ramiro Barrantes-Reynolds, Pawel Jaruga, Michael Newton, Cynthia Burrows, Viswanath Bandaru, Miral M. Dizdar, Jeffrey Bond, Susan Wallace
Formamidopyrimidine DNA glycosylase (Fpg) and endonuclease VIII (Nei) share an overall common three dimensional structure and primary amino acid sequence in conserved structural motifs but have different substrate specificities, with bacterial Fpg proteins

Nanofluidic Structures with Complex Three Dimensional Surfaces

March 31, 2009
Samuel M. Stavis, Elizabeth Strychalski, Michael Gaitan
A fabrication process was developed to construct nanofluidic devices with complex three dimensional (3D) topographies. A single layer of grayscale photolithography enabled arbitrary and simultaneous control of numerous nanoscale etch depths in a fused

Measurement of formamidopyrimidines in DNA

December 15, 2008
Pawel Jaruga, Guldal Kirkali, Miral M. Dizdar
Formamidopyrimidines, 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua), are among major lesions in DNA generated by hydroxyl radical attack, UV radiation or photosensitization in vitro and in vivo

Demonstration of Rapid Multiplex PCR Amplification Involving 16 Genetic Loci

December 1, 2008
Peter Vallone, Carolyn R. Steffen, John M. Butler
Current forensic DNA typing is conducted in approximately eight to ten hours with steps including DNA extraction, quantitation, polymerase chain reaction (PCR) amplification of multiple short tandem repeat (STR) loci, capillary electrophoresis separation

Addressing Y-Chromosome Short Tandem Repeat(Y-STR) Allele Nomenclature

November 25, 2008
John M. Butler, Margaret C. Kline, Amy E. Decker
Currently 120 different Y-chromosome short tandem repeat (Y-STR) markers are used by various genetic genealogy testing laboratories. In some cases, different laboratories may designate the same Y-STR allele with two different nomenclatures making data

Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

January 25, 2008
Carolyn R. Steffen, Margaret C. Kline, Michael Coble, John M. Butler
An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized to aid in the analysis of degraded DNA samples. These new markers produce short PCR products in the target range of 50 150 base pairs (bp) by moving the

Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits

July 25, 2007
Carolyn R. Steffen, Margaret C. Kline, Julio J. Mulero, Robert E. Lagace, Chien-Wei Chang, Lori K. Hennessy, John M. Butler
The AmpFlSTR MiniFiler PCR Amplification kit developed by Applied Biosystems enables size reduction on eight of the larger short tandem repeat (STR) loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA

Analysis of artificially degraded DNA using STRs and SNPs results of a collaborative European (EDNAP) exercise

December 1, 2006
L. A. Dixon, A. E. Dobbins, H. Pulker, John M. Butler, Peter M. Vallone, Michael D. Coble, W. Parson, B. Berger, P. Grubwieser, H. S. Mogensen, N. Morling, K. Nielsen, J. J. Sanchez, E. Petkovski, A. Carracedo, P. Sanchez-Diz, E. Ramos-Luis, M. Brion, J. A. Irwin, R. S. Just, O. Loreille, T. J. Parsons, D. Syndercombe-Court, H. Schmitter, B. Stradmann-Bellinghausen, K. Bender, P. Gill

Analysis of potential cancer biomarkers in mitochondrial DNA

December 1, 2006
J P. Jakupciak, G. D. Dakubo, Samantha D. Maragh, R. L. Parr
Understanding mitochondrial biology is a fundamental research goal in human genetics and medicine. The use of mitochondria to serve as a biomarker is rapidly expanding in disciplines ranging from cancer, rare metabolic diseases, aging, the tracing of human
Displaying 76 - 100 of 233