We report on temperature time domain and spectral measurements for on-chip microwave thermocycling. In particular we observe enhanced specificity of the heating of a targeted sample compared to conventional heating block-based thermocyclers. Spatial control of the temperature in the device is implemented by delivering microwave energy to specific locations in the microfluidic chip. Our device characterization shows a linear response between the rise in temperature and the applied power as predicted by theory. On-chip thermocycling by microwave dielectric heating is characterized and compared to a commercial heating block-based thermocycler. This method is applicable to any reaction where fast and accurate heat transfer is required. We are currently developing PCR-based applications based on this technique.
Proceedings Title: The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS)
Conference Dates: November 5-9, 2006
Conference Location: Tokyo, JA
Pub Type: Conferences
dielectric heating, DNA, microwave, PCR, thermocycling