In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 catalytic subunit alone and the Tk p41-p46 complex in the absence of a DNA template. They were not formed with preparations containing the catalytically inactive p41 subunit. Similar glycerol and Tris derivative were also formed with dGTP, dCTP or dTTP. In addition to these derivatives, dNMPs were also formed in these reactions. The mechanism contributing to the formation of these products is discussed.
Citation: Journal of Biological Chemistry
Pub Type: Journals
initiation reactions, nucleotide adducts, template independent