The goal of this project is to provide an SI-traceable reference material for use by calibrant and reagent manufacturers in production of their own calibrants and standards. Clinical laboratories with in-house methods will be able to use the reference material for production of their own quality control materials, which will be traceable to a NIST standard.
Whole BKV or JCV virus genomes will be inserted into a cloning vector, propagated in Escherichia coli, extracted, linearized, and certified for number of copies of the genome per volume (e.g. copies per microliter). The DNA will be sequenced and provided as information value in the certificate of analysis.