Jeff Stinson joins NIST as an NRC Postdoctoral Research Associate working with Michael Halter in the Cell Systems Science Group. His current research involves attempting to develop standardized methods for induced pluripotent stem cell (iPSC) derived cardiomyocyte differentiation using geometric patterning. The use of patterning should reduce the iPSC colony-to-colony variation and subsequent differentiation. This reduction in cellular response “noise” will allow induced system perturbations to identify and characterize critical measurable parameters that impact levels of cellular heterogeneity that arise during differentiation. These will be measured using live cell, quantitative phase contrast and fluorescence microscopy techniques. In an earlier life, Jeff worked in biotech and biopharma for about 20 years, mostly in small start-up environments focused on recombinant protein-based therapeutic development (antibodies, enzymes, peptides), spending his last two years as Director of Molecular Engineering and Director of Quality Control. He subsequently returned to academe obtaining his PhD in Cell and Molecular Biology from the Uniformed Services University for the Health Sciences (Bethesda, MD) in 2015. His dissertation and post-doctoral research investigated alterations in the NF-kB signal transduction pathways in B and T cells caused by gain-of-function mutations in CARD11, a critical scaffolding protein that regulates lymphocyte response to antigen. This work confirmed the genetic cause of a newly identified lymphoproliferative disease, B cell Expansion with NF-kB and T cell Anergy (BENTA).
Ma CA, Stinson JR, Zhang Y, Abbott JK, Weinreich MA, Hauk PJ, Reynolds PR, Lyons JJ, Nelson CG, Ruffo E, Dorjbal B, Glauzy S, Stoddard J, Niemela J, Rosenzweig SD, McElwee JJ, DiMaggio T, Stone KD, Palma A, Oleastro M, Prieto E, Bernasconi A, Dubra G, Danielian S, Zaiat J, Marti M, Kim B, Cooper MA, Romberg N, Meffre E, Gelfand EW, Snow AL, Milner JD (2016). Germline hypomorphic CARD11 mutations in severe atopic disease. Nat Gen, in review.
Sasha E. Larsen, Abegail Bilenkin, Tatiana N. Tarasenko, Swadhinya Arjunaraja, Jeffrey R. Stinson, Peter J. McGuire and Andrew L. Snow (2016). Sensitivity to restimulation-induced cell death is linked to glycolytic metabolism in human T cells. J Immunol, ePub, Nov 16.
Jeffrey R. Stinson, Dennis McDaniel, Liron David, Hao Wu, & Andrew L. Snow (2015) Spontaneous aggregation and novel signalosome organization of gain-of-function CARD11 mutants in lymphocytes. Mol Cell Biol (in revision)
David Buchbinder*; Jeffrey R Stinson*, Diane J. Nugent, Helen Su, Clifton Dalgard, Gauthaman Sukumar, Yu Zhang, Andrew L. Snow (2015) A Novel Gain-of-Function Mutation in the CARD Domain of CARD11 (C49Y) Results in BENTA Disease. J Allergy and Clin Immunol. 2015. 136(3): 819-821 (*co-first authorship)
Brohl AS*, Stinson JR*, Su HC, Badgett T, Jennings CD, Sukumar G, Sindiri S, Wang W, Kardava L, Moir S, Dalgard CL, Moscow JA, Khan J, Snow AL. (2014) Germline CARD11 Mutation in a Patient with Severe Congenital B Cell Lymphocytosis. J Clin Immunol. 2014. Oct 29 35:32-46 (*co-first authorship)
Gil Katz, Scott M. Krummey, Sasha E. Larsen, Jeffrey R. Stinson, and Andrew L. Snow (2014) SAP Facilitates Recruitment and Activation of LCK at NTB-A Receptors during Restimulation-Induced Cell Death. J Immunol 2014. 192:4202-4209
Snow A.L., W. Xiao, J. R. Stinson, B. Chaigne-Delalande, S. Pittaluga, H. F. Matthews, W. Lu, L. Zheng, R. Schmitz, S. Jhavar, S. Kuchen, I. T. Lamborn, H. Jing, M. Raffeld, H. C. Su, L. M. Staudt, and M. J. Lenardo (2012) Congenital B cell lymphocytosis explained by novel germline CARD11 mutations. J Exp Med 2012. 209(12): p. 2247-61
7,884,198 Polynucleotides encoding humanized and chimeric antibodies specific for lipoteichoic acid of gram-positive bacteria
7,777,017 Nucleic acids encoding opsonic monoclonal and chimeric antibodies specific for lipoteichoic acid of gram-positive bacteria
7,511,122 Humanized and chimeric antibodies specific for lipoteichoic acid of gram-positive bacteria
7,250,494 Opsonic monoclonal and chimeric antibodies specific for lipoteichoic acid of gram-positive bacteria
7,169,903 Multifunctional monoclonal antibodies directed to peptidoglycan of gram-positive bacteria
6,939,543 Opsonic and protective monoclonal and chimeric antibodies specific for lipoteichoic acid of gram-positive bacteria
6,610,293 Opsonic and protective monoclonal and chimeric antibodies specific for lipoteichoic acid of gram-positive bacteria
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5,767,369 DNA sequences encoding SAR8.2 proteins and uses thereof
5,650,505 Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
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