Previously, we demonstrated liposome formation using hydrodynamic focusing in a microfluidic network which enables us to produce liposomes of a controlled size and size distribution in a reproducible fashion . Former measurements on liposomes were conducted with batch dynamic light scattering (DLS) and multi angle laser light scattering (MALLS) of unfractionated samples. However, this measurement only provides ensemble averages. In this paper, a more detailed and accurate measurement technique that allows for precise characterization of liposome populations using asymmetric flow field flow fractionation (AFFFF) before MALLS and DLS is shown. Details about liposome size and size distribution allow further elucidation about the liposome self-assembly process.
Proceedings Title: Proceedings of the 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)
Conference Dates: November 5-9, 2006
Conference Location: Tokyo, JA
Conference Title: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)
Pub Type: Conferences
flow field flow fractionation, liposomes, nanoparticle, self-assembly