It is well known that an intimate relationship exists between vascularization and bone formation during development and fracture healing, where new bone formation is preceded by local vascularization. A recent rat exercise model has demonstrated that mechanical loading induced increases in bone formation are also preceded by increased bone vascularization, and correlated to blood vessel number. However, the cellular cues that lead to increased or maintained vascularization of bone remain unclear. Specifically, it is not clear whether osteoblasts, osteocytes (OCY), and/or osteoclasts are involved in the recruitment of new vasculature. Since OCYs form extensive interconnected cellular networks throughout the bone tissue and respond to a variety of mechanical and chemical stimuli, they have been proposed to be the mechano/chemostat cells of bone tissue. Along these lines, osteoporotic bone, which has decreased OCY density and a disorganized osteocyte network, has reduced number of capillaries and fewer vessels closely apposed to the bone surface. Thus OCYs may play an important role in vascular recruitment both with and without mechanical loading. In this study we have examined the ability of OCYs subjected to various physiologic stimuli to enhance angiogenesis in vitro.
Proceedings Title: Annual Meeting of the Orthopaedic Research Society | 53rd | | Orthopaedic Research Society
Conference Dates: February 11-14, 2007
Conference Title: Transactions of Annual Meeting of the Orthopaedic Research Society
Pub Type: Conferences
angiogenesis, bone, co-culture, endothelial cell, mechanotransduction, osteocycle