A combinatorial method to rapidly screen cell-material interactions in three-dimensional (3-D) culture format was developed for accelerating tissue engineering research. Hydrogels of poly(ethylene glycol) dimethacrylate (PEGDM) with gradients in compressive modulus were fabricated using this platform. The effect of modulus on encapsulated murine preosteoblast MC3T3-E1 cells was investigated as a model for orthopedic tissue engineering. Cell survival was found to be higher in the softer ends of the gradients, but increase in gel stiffness induced spontaneous cell differentiation. Prolonged cell culture led to the formation of gradients of mineral deposits. These graded tissues can also be applied for seamless interfacing of tissue-engineered mineralized bones with softer tissues. These results demonstrate the feasibility of the gradient platform and offer a more relevant 3D method for rapid screening of cell-biomaterial interactions.
Proceedings Title: 238th ACS National Meeting & Exposition, Fall 2009
Conference Dates: August 16-20, 2009
Conference Location: Washington, DC
Pub Type: Conferences
combinatorial methods, gradient, scaffold, polymer, tissue engineering, biomaterial, cell differentiation, osteoblast, bone, hydrogel, modulus