Utilizing tissue scaffold architecture to induce stem cell differentiation is of tremendous importance to the regenerative medicine community. To this end, we have investigated the effect of nanofiber scaffold structure on primary human bone marrow stromal cell (hBMSC) differentiation. hBMSC function was tested on biocompatible poly(-caprolactone) (PCL) and poly(DL-lactic acid) (PDLLA) nanofibers. Flat spun-coat surfaces of the same materials were used as controls. Since cell differentiation is tightly linked to cell morphology, we used high resolution confocal microscopy to measure and compare cell morphology on PCL nanofibers and films. Further, we have compared cellular morphologies during osteogenesis driven by nanofibers to that driven by osteogenic supplements (OS). We find that OS induce hBMSCs to adopt morphologies similar to that found on nanofibers. These results indicate that nanofiber scaffold architecture induces hBMSCs to adopt morphologies associated with osteogenic differentiation.
Conference Dates: April 13-16, 2011
Conference Location: Orlando, FL
Conference Title: Society for Biomaterials
Pub Type: Conferences
nanofibers, polymer scaffold, stem cell, osteogenesis, cell morphology, 3D imaging