GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES

Published

October 28, 2012

Author(s)

Conni Hanke, Petra S. Dittrich, Darwin Reyes-Hernandez

Abstract

A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cells were trapped underneath the PET membrane (bottom channel) when DEP attractive forces were applied. Cells immediately adhered on the PET membrane by the electrostatic forces from a hybrid cell adhesive material (hCAM) deposited on the surface of the electrodes. NIH-3T3 cells adhered on the top channel via the hCAM deposited on that side of the membrane. Cells on both sides of the membrane remained attached and viable at least 24 h after the co-culture was generated.

Proceedings Title

Proceedings of Micro Total Analysis Systems 2012

Conference Dates

October 28-November 1, 2012

Conference Location

Okinawa, JP

Conference Title

The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Pub Type

Conferences

Keywords

Dielectrophoresis, cell co-cultures, polyester (PET) membrane, fibronectin, polyelectrolyte multilayers

Citation

Hanke, C. , Dittrich, P. and Reyes-Hernandez, D. (2012), GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES, Proceedings of Micro Total Analysis Systems 2012, Okinawa, JP (Accessed April 18, 2024)