We demonstrate a versatile, well controlled, and highly reproducible surface density gradient fabrication strategy that allows for precise control over surface functionality and slope. Our approach involves of a novel vacuum away channel confinement vapor deposition method, which overcomes challenges with regard to chemical tunability concentration control and has potential for translation into complicated geometries that are present in the aforementioned gradient formation methods. ESC differentiation results illustrate how amine concentration can affect cellular function and provides new information on the optimal therapeutic range of amines on ESC neural differentiation while providing a novel platform to study the variations affect neural differentiation and warrant further investigation. Future studies will translate these findings to clinically relevant substrates.
Citation: Biomaterials Science
Pub Type: Journals
monolayers, self-assembly, gradients, differentiation, cells, surfaces