Standard Reference Material (SRM) 2944 is a cuvette-shaped, Bi-ion-doped glass, recommended for use for relative spectral correction of emission and day-to-day performance verification of steady-state fluorescence spectrometers. Properties of this standard that influence its effective use or contribute to the uncertainty in its certified emission spectrum were explored here. These properties include its photostability, absorbance, dissolution rate in water, anisotropy and temperature coefficient of fluorescence intensity. The expanded uncertainties in the certified spectrum are about 4 % around the nominal peak maximum at 704 nm, using an excitation wavelength of 515 nm.
Citation: Journal of Luminescence
Pub Type: Journals
fluorescence, instrument qualification, Bi glass, spectral correction, SRM, standards