A microfabricated device comprised of a microelectrode array (MEA) and a microfluidic channel is presented here for the purpose of trapping cells using positive dielectrophoresis (DEP). Transparent indium tin oxide (ITO) electrodes are patterned in an array of electrode pairs. A microfluidic channel made up of polydimethylsiloxane (PDMS) is then attached on top of the electrode array. DEP is used to trap P19 cells at specific positions on the ITO electrode array within the PDMS channel. Our method provides exact positioning of cells and better cell access. We show here the design and results on cell trapping with this novel microelectrode array.
Proceedings Title: IEEE Engineering in Medicine and Biology Society Neural Engineering Conference Proceedings
Conference Dates: November 6-8, 2013
Conference Location: San Diego, CA
Conference Title: The 6th International IEEE EMBS Neural Engineering Conference
Pub Type: Conferences
dielectrophoresis, P19 cells, microelectrode arrays, hybrid cell adhesive material (hCAM)