Carbapenems are broad spectrum antibiotics considered as last resort drugs to treat bacterial infections. Carbapenem-hydrolyzing β-lactamases (also called carbapenemases), however, can confer bacterial resistance and represent a serious health threat. Here, we report a novel approach using 18O labeling and selected reaction monitoring to detect carbapenemase activity from pathogenic microorganisms in a rapid and quantitative manner. Four model bacterial strains bearing various classes of β-lactamases were tested for their capability to hydrolyze meropenem, a FDA-approved carbapenem drug. We were able to predict the meropenem resistance of these bacteria based upon their carbapenemase activity, suggesting the great potential of our method in clinical diagnostics.
Citation: Analytical Chemistry
Pub Type: Journals
carbapenemase, selected reaction monitoring, isotope labelling, mass spectrometry