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Surface Desorption Ambient Mass Spectrometry

Surface desorption ambient mass spectrometry enables direct solid surface interrogation for sampling and ionization of trace analytes. This project encompasses liquid-, plasma-, and laser-based ion sources. These versatile ion source enable direct analysis of samples in situ, requiring minimal or no sample preparation. Real-time surface sampling also enables measurement of the spatial and temporal distribution of chemical species on surfaces under ambient conditions.   

The STCAG maintains a range of research avenues into the characterization, analysis, and optimization of various commercial and in-house developed surface desorption ion sources. These research avenues also aim to advance our fundamental understanding of the physicochemical processes involved as well as application specific optimization and implementation. Below are a few examples of research conducted in these areas.  

The ambient ion source desorption electro-flow focusing ionization (DEFFI) couples electro-flow focusing in a surface desorption configuration, similar to desorption electrospray ionization (DESI). DEFFI incorporates a concentric laminar gas flow that focuses a solvent stream through a small orifice from a recessed capillary. This configuration achieves a steady jet of micron sized droplets that can be charged through the application of an electric field. DEFFI enables stable jetting at low applied pressures and ionization regimes dominated by charged-droplet electrospraying or corona discharge chemical ionization. The DEFFI surface analysis ion source has been applied to the trace detection of narcotics, explosives, and elemental inorganics for applications including homeland security and forensic chemistry. The fine jetting produced by DEFFI was also applied to the chemical imaging of endogenous and exogenous components from artificial fingerprints as well as investigating chemical spatial distributions from wipe-collected analytes.

Ambient Mass Spec
Figure A. (left) Schematic representation of desorption electro-flow focusing ionization (DEFFI). (right) Mass spectrometry images of endogenous and exogenous compounds from artificial fingerprints on forensic lift tape (lighter color indicates higher relative intensity).  

 

Another front-end source for ambient MS is the low temperature plasma (LTP). By passing electricity through a small volume of helium gas flowing in a tube, a filamentary discharge of metastable helium atoms can be created (Figure B). This filamentary discharge is used to probe the sample surface, from which molecules are desorbed and ionized for analysis inside a MS system. LTP can rapidly etch through the sample, and is a very destructive form of a material desorption source. It is very suited for inorganic material analysis such as metal particles, as a source of fluorine such as CF4 and SF6 gas can be mixed with helium to promote the metals to form volatile tetrafluoride and hexafluoride complexes, though it is limited to the analysis of metals that can form such complexes (Figure C).

Ambient Mass Spec
Figure B. (a) Schematic of the low temperature plasma probe, showing the position of the MS inlet with respect to the filamentary plasma jet. (b) The optical micrograph shows a very visible plasma discharge in contact with the sample.

Ambient Mass Spec
Figure C. An example of metal etching using LTP. A multilayer made of very thin layers of silicon (10 nm) sandwiched between tungsten layers (50 nm) was etched with LTP using a helium/SF6 mixture gas. The resulting crater was visualized using an imaging MS (ToF-SIMS) to reveal layers of silicon embedded within a tungsten film.

 

Direct analysis in real time (DART) is a commercial ion source that has experienced considerable interest and development as an analytical tool for the forensic science community. DART incorporates a heated gas stream for analytes desorption, followed by interaction with ions generated by the emission of helium metastables. An off-axis geometry for surface desorption was implemented for an investigation into the detection of sugar alcohol precursors, by-products, and corresponding nitrate ester explosives. These homemade explosives are synthesized through the nitration of readily available sugar alcohols (commonly found as artificial and alternative sweeteners). DART-MS demonstrated sensitive detection of the nitrate ester explosives from mixtures containing precursors and potential by-products. 

Ambient Mass Spec
Figure D. Schematic representation of surface desorption DART-MS. Representative mass spectrum for possible mixture of synthesis components and nitrate ester explosive.

 

Created August 15, 2017, Updated November 15, 2019