Fluorescence In Situ Hybridization Using Semiconductor Nanocrystal Probes in a
The technique known as fluorescence in situ hybridization (FISH) is routinely used in gene research and clinical applications. Fluorophore-labeled DNA probes with a specific and complementary sequence is allowed to intercalate into a gene of interest for detection and/or quantitation. Typically used organic labels, such as Texas Red or fluorescein, are prone to bleaching when utilized for FISH experiments. Recently, the compatibility of semiconductor nanocrystals, or quantum dots, with biological systems has been greatly improved. Quantum dot labeling of DNA hybridized probes offers a brighter, more stable fluorescence-label for gene research when compared to often-employed organic fluorophores.
Figure 1. Image of total DNA within cells by utilizing FISH with quantum dot fluorophores performed in a microfluidic channel
J. Christopher Ball
Chemical Science and Technology Laboratory
Bldg 227 A361
Sigma Xi member- NO
Poster Category: Chemistry